Xanthan gum is an extracellular hetero polysaccharides of many industrial applications and produced by Xanthomonas campestris. This biopolymer is produced as extracellular product in submerged fermentation system. The production of xanthan was influenced by the concentration in glucose in medium. The maximal volumetric production of 17.9 g L was obtained in 80 g L glucose culture in shake flask...

Batch and fed-batch cultivation of Bacillus thurillgiellsis var. kurstaki was carried out to achieve high cell and spore density with maximum insecticidal activity. Cell cultivation was done in fully equipped fermentors under controlled conditions at different feed rates. Increased cell mass, high spore counts and improved insecticidal activity were achieved with fed-batch cultivation system. W...

Most large-scale production processes in biotechnology are performed in fed-batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode, which results in the microorganisms being subjected to different physiological conditions. This significantly affects strain selection. To demonstrate differences in ranking during strain selection depending on th...

BACKGROUND The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted...

OBJECTIVES Recombinant protein production processes in Escherichia coli are usually operated in fed-batch mode; therefore, the elaboration of a fed-batch cultivation protocol in microtiter plates that allows for screening under production like conditions is particularly appealing. RESULTS A highly reproducible fed-batch like microtiter plate cultivation protocol for E. coli in a micro-bioreac...

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase p...

First report on the production of protease through fed batch cultivation using co-culture of Pseudomonas putida and Staphylococcus aureus from a crude carbon source, banana peel. Batch production by using synthetic medium, impact of some of the process parameters on cell growth and alkaline protease activity was investigated and the optimized values were found to be inoculum size of 5 % (v/v), ...

Biohydrogen fermentation using immobilized cells of Thermotoga neapolitana on porous glass beads was successfully performed in a continuously stirring anaerobic bioreactor (CSABR) system operated under the conditions of temperature 75 o C, pH 7.0 and 5.0 g/L pentose (xylose) and/or hexose (glucose). The results showed that both batch and fed-batch cultivations of the immobilized cells were effe...

BACKGROUND RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the viral gene. In this study, we employed a combinatorial approach to interfere multiple gene functions of white spot syndrome virus (WSSV), the most lethal shrimp virus, using a single-batch of dsRNA, so-called "multi-WSSV dsRNA." A co-culti...