background:   objective: the aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ala).   materials and methods: pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified–warmed pre-antral fol...

Effect of co-culture of buffalo preantral follicles with different somatic cells, i.e. cumulus cells, granulosa cells, Ovarian Mesenchymal Cells (OMC) and Oviductal Epithelial Cells (OEC) was studied. Large preantral follicles were isolated by microdissecting the trypsin (1%) digested ovarian cortical slices. Cumulus cells were isolated by repeated pipetting of oocytes, granulosa cells were iso...

Background: The aim of the present study was to develop a three-dimensional (3D) collagen gel culture system for the in vitro growth and survival of buffalo preantral follicles with or without growth factors. Materials and Methods: Buffalo ovaries were collected from a local abattoir and preantral follicles were isolated through microdissection. Isolated preantral follicles were put either in c...

Localization of androgen receptor (AR) was investigated in ovarian follicles developing and undergoing atresia during pregnancy in the pig. Immunohistochemical staining was conducted on ovarian antral follicles isolated on different days of gestation: 10, 18, 32, 50, 70, and 90. Paraffin sections were also subjected to in situ DNA labeling. TUNEL staining revealed the presence of positive folli...

occurrenceofoxidative stress (os) following in vitro culture is inescapable. therefore, the aim of the present study was to determine the efficacy of coenzyme q 10 (coq 10 ) supplementation medium on developments and total antioxidant capacity (tac) of mouse vitrified pre-antral follicles. fresh and vitrified-warmed isolated preantral follicles from ovaries of 14-16 day s -old mice were cultured...

Background: Recent improvement in treatments of cancer has significantly increased survival rates of cancer patients. Unfortunately these treatments such as chemotherapy and radiotherapy can sever damage to the ovaries. Ovarian cryopreservation prior to chemo/ radiotherapy and auto-transplantation post-treatment can restore fertility to cancer patients. Hypoxia in the transplant from delayed re...

This study aimed to investigate the time course of disappearance of the mRNAs of the various subunits of inhibin in follicles which become atretic. An animal model was used in which atresia of preovulatory follicles could be studied in a chronological order. Injection of gonadotrophin-releasing hormone (GnRH) antagonist (20 microg) at the morning of pro-oestrus (P) blocked ovulation and the 10-...