نتایج جستجو برای: NSP4

تعداد نتایج: 349  

Background: Rotavirus group A (RVA) is recognized as a major cause of severe gastroenteritis in children and new-born animals. Nonstructural protein 4 (NSP4) is responsible for the enterotoxic activity of these viruses in the villus epithelial cells. Amino acids 114-135 of NSP4 are known to form the diarrhea-inducing region of this viral enterotoxin. Therefore, developing an NSP4 lacking the en...

Journal: :medical journal of islamic republic of iran 0
z sharifi 123from the virology department, iran university of medical sciences, tehran, the national research center for genetic engineering and biotechnology (nrcgeb), tehran, and the iranian blood transfusion organization, tehran, iran, b yakhchali national research center for genetic engineering and biotechnology (nrcgeb), tehran ms shahrabadi from the virology department, iran university of medical sciences, tehran

rotavirus nonstructural glycoprotein nsp4 can induce diarrhea in newborn mice. it has been suggested that nsp4 may be a key determinant for rotavirus pathogenesis and a target for vaccine development. in order to study the biological and morphological role of nsp4 a large amount of the purified protein and antibody against it are required. simian rotavirus sa11 was propagated in bscl cell, puri...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1991
R J de Groot T Rümenapf R J Kuhn E G Strauss J H Strauss

Upon infection of animal cells by Sindbis virus, four nonstructural (ns) proteins, termed nsP1-4 in order from 5' to 3' in the genome, are produced by posttranslational cleavage of a polyprotein. nsP4 is believed to function as the viral RNA polymerase and is short-lived in infected cells. We show here that nsP4 produced in reticulocyte lysates is degraded by the N-end rule pathway, one ubiquit...

Journal: :Journal of virology 2006
Rebecca D Parr Stephen M Storey Deanne M Mitchell Avery L McIntosh Minglong Zhou Kiran D Mir Judith M Ball

Rotavirus nonstructural protein 4 (NSP4) is known to function as an intracellular receptor at the endoplasmic reticulum (ER) critical to viral morphogenesis and is the first characterized viral enterotoxin. Exogenously added NSP4 induces diarrhea in rodent pups and stimulates secretory chloride currents across intestinal segments as measured in Ussing chambers. Circular dichroism studies furthe...

Journal: :American journal of physiology. Gastrointestinal and liver physiology 2014
Shuaiyu Zheng Hongyi Zhang Xiaojin Zhang Fei Peng Xuyong Chen Jixin Yang David Brigstock Jiexiong Feng

Interferon (IFN)-γ-driven and CD8+ T cell-dependent inflammatory injury to extrahepatic biliary epithelium (EHBE) is likely to be involved in the development of biliary atresia (BA). We previously showed that viral protein NSP4 is the pathogenic immunogen that causes biliary injury in BA. In this study, NSP4 or four synthetic NSP4 (NSP4(157-170), NSP4(144-152), NSP4(93-110), NSP4(24-32)) identi...

2010
Joseph M. Hyser Matthew R. Collinson-Pautz Budi Utama Mary K. Estes

Many viruses alter intracellular calcium homeostasis. The rotavirus nonstructural protein 4 (NSP4), an endoplasmic reticulum (ER) transmembrane glycoprotein, increases intracellular levels of cytoplasmic Ca(2+) ([Ca(2+)]cyto) through a phospholipase C-independent pathway, which is required for virus replication and morphogenesis. However, the NSP4 domain and mechanism that increases [Ca(2+)]cyt...

Journal: :Journal of virology 2000
M Zhang C Q Zeng A P Morris M K Estes

Previous studies have shown that the nonstructural glycoprotein NSP4 plays a role in rotavirus pathogenesis by functioning as an enterotoxin. One prediction of the mechanism of action of this enterotoxin was that it is secreted from virus-infected cells. In this study, the media of cultured (i) insect cells infected with a recombinant baculovirus expressing NSP4, (ii) monkey kidney (MA104) cell...

Journal: :Clinical and diagnostic laboratory immunology 2005
Esmeralda Vizzi Eva Calviño Rosabel González Irene Pérez-Schael Max Ciarlet Gagandeep Kang Mary K Estes Ferdinando Liprandi Juan E Ludert

The immune response elicited by the rotavirus nonstructural protein NSP4 and its potential role in protection against rotavirus disease are not well understood. We investigated the serological response to NSP4 and its correlation with disease protection in sera from 110 children suffering acute diarrhea, associated or not with rotavirus, and from 26 children who were recipients of the rhesus ro...

Journal: :Journal of virology 1997
R L Ward B B Mason D I Bernstein D S Sander V E Smith G A Zandle R S Rappaport

The NSP4 protein of a simian rotavirus was reported to induce diarrhea following inoculation of mice. If NSP4 is responsible for rotavirus diarrhea in humans, attenuation of a human rotavirus may be reflected in concomitant mutations in the NSP4 gene. After 33 passages in cultured monkey kidney cells, a virulent human rotavirus (strain 89-12) was found to be attenuated in adults, children, and ...

Journal: :Memorias do Instituto Oswaldo Cruz 2008
Talissa de Moraes Tavares Wilia Marta Elsner Diederichsen de Brito Fabíola Souza Fiaccadori Erika Regina Leal de Freitas Juliana Alves Parente Paulo Sérgio Sucasas da Costa Loreny Gimenes Giugliano Márcia Sueli Assis Andreasi Célia Maria Almeida Soares Divina das Dôres de Paula Cardoso

Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of differen...

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