Radioactive end-labeling is useful for visualizing and allowing the detection of nucleic acids at trace concentrations. Radioactive end-labeling can be carried out on RNA, DNA, or other modified nucleic acids. For RNA, the uses of end-labeling extend beyond simple detection of the intact RNA. A number of RNA molecules studied by biologists form three-dimensional structures in solution, and many...

Novel synthetic methods for producing an array of chelates for use in "click"-radiolabeling of peptides are described, and their reactivity with regards to subsequent conjugation and radiolabeling is discussed.

These studies focused on a new radiolabeling technique with copper ((64)Cu) and zirconium ((89)Zr) for positron emission tomography (PET) imaging using a CD45 antibody. Synthesis of (64)Cu-CD45 and (89)Zr-CD45 immunoconjugates was performed and the evaluation of the potential toxicity of radiolabeling human peripheral blood stem cells (hPBSC) was assessed in vitro (viability, population doublin...

In recent years, the diagnostic and therapeutic uses of radioisotopes have shown significant progress. Immunoglobulin (Ig) appears to be a promising tracer, particularly due to its ability to target selected antigens. The main objective of this study is to optimize and assess an Ig radiolabeling method with Technetium 99m (99mTc), an attractive radioelement used widely for diagnostic imaging. M...

Polyphosphate grafted Fe 3 O 4 nanomagnets for pH-responsive drug delivery and their in vivo tracking by 64 Cu 177 Lu radiolabeling.

introduction: due to the anti-proliferative properties of platinum group-thiosemicarbazone complexes, the production of 191os-labeled 2-acetyl pyridine 4-n-methylthiosemicarbazone (191os-apmts) was investigated. methods: [191osmium (t½= 15.4d) was produced via the 190os(n,γ)191os nuclear reaction using enriched target irradiated with thermal neutrons. reaction of in-house synthesized 2-acetylpy...

Correction for ‘Radiolabeling and in vivo evaluation of lanmodulin with biomedically relevant lanthanide isotopes’ by Kirsten E. Martin et al. , RSC Chem. Biol. 2023, https://doi.org/10.1039/D3CB00020F.

The most widely cited procedures for radiolabeling antibodies with zirconium-89 for immuno-PET require multi-milligram amounts of antibody which can be cost-prohibitive, particularly during the research and development process. We therefore sought to develop a reliable (89)Zr-radiolabeling procedure that provides high radiochemical yields at the microgram scale.

The radiolabeling of antibodies is considered in terms of the choice of radionuclide, the method of conjugation, and the effect of conjugation on plasma clearance, lodination tech niques are reviewed, but the major emphasis is placed on the methods of conjugating metallic radionuclides using bifunctional chelating agents. The technique of producing clinically useful radiolabeled antibodies by b...