Moloney murine leukemia virus reverse transcriptase (MMLV-RT) is a widely used enzyme for cDNA synthesis. Here we show that MMLV-RT has a strong template-independent polymerase activity using blunt DNA ends as substrate that generates 3' overhangs of A, C, G, or T. Nucleotides were appended efficiently in the order A > G > T > C, and tail lengths varied from 4 to 5, 2 to 7, 2 to 4, and 2 to 3 f...

Sulfide lead resources are being depleted and the exploitation of carbonate lead deposits is now the main focus of lead mining. Cerussite, PbCO3, is majorly discarded to tailing damps because it is difficult to be processed by flotation in lead concentration units. This paper not only investigates the optimization of cerussite flotation, but it also proposes a model for predict...

agh darreh tailing dam located in 23 km north of takab, west azarbayjan province is used for disposal of mine tailings. the tailing dam and its reservoir were constructed on gypsiferous marlstones. this is emphasis on geotechnical and environmental importance of the site. although construction of dams on gypsum rocks were not yet prohibited, but the dissolution of gypsum in contact with water i...

The stability of tailing dam has an important influence on the surrounding ecological environment and the security and property of people. This article, from the micro perspective, analyzes of the water-tailing chemical interactions and process and analyzes its effects on tailing micro structure and dam stability. The experimental results show that ferrous iron in water(PH=9.5-10.3) is constant...

The failure of a tailing dam occurs due to damage to the particles’ micro-structure. Understanding the deformation characteristics of the particle’ micro-structure is important for understanding the mechanics of instability in tailing dams. In our study, a series of experiments was conducted using a testing apparatus for micro-mechanics and the deformation of tailings from the Huangcaoping tail...

To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of polymerase chain reaction (PCR) primer sequences. The RNA pool tested was extracted from marine s...

This study investigates the mechanisms underlying the deviation from Chick-Watson kinetics, namely a tailing curve, during the disinfection of viruses by chlorine dioxide (ClO2). Tailing has been previously reported, but is typically attributed to the decay in disinfectant concentration. Herein, it is shown that tailing occurs even at constant ClO2 concentrations. Four working hypothesis to exp...