نتایج جستجو برای: Transfection assay

تعداد نتایج: 239598  

Journal: :jundishapur journal of microbiology 0
ali teimoori department of virology, faculty of medical sciences, tarbiat modares university, tehran, ir iran hoorieh soleimanjahi department of virology, faculty of medical sciences, tarbiat modares university, tehran, ir iran; department of virology, faculty of medical science, tarbiat modares university, tehran, ir iran. tel/fax: +98-2182883561 manoochehr makvandi department of virology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

conclusions therefore the non-infectious dlps were became infectious if introduced into the cytoplasm of permissive and cancerous cells, without passing attachment and entry process. results we attempt to avoid the attachment and entry of the rotavirus by using lipofectamine to mediate the delivery of viral particles directly into the cytoplasm. dlp was endocytosed into the cytoplasm following ...

Journal: :iranian journal of public health 0
mansoor aletaha behzad mansoori ali mohammadi mehdi fazeli behzad baradaran

background: breast cancer is the most common diagnosed cancer among women in the world. snail1 plays a role in the development of the invasive phenotypes of cancer, neural cell differentiation, cell division and apoptosis in tumor cells. traces of snail1 in metastasis of breast cancer to bone are observed. the aim of this study was to investigate the effect of specific snail1 sirnas on the prol...

Background: Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ab...

Journal: :iranian journal of basic medical sciences 0
reza k. oskuee department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran ali dehshahri department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran wayne. t. shier department of medicinal chemistry, university of minnesota-twin cities, minneapolis, mn 55455, usa mohammad ramezani department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran

objective polyethylenimine (pei), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. but it has been reported that pei is cytotoxic in many cell lines. in this study, in order to enhance the transfection efficiency of 10 kda pei and reduce its toxicity, hydrophobic residues were grafted...

Journal: :iranian journal of basic medical sciences 0
faezeh moghadam ariaee pharmaceutical research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran maryam hashemi nanotechnology research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran sara amel farzad pharmaceutical research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran khalil abnous pharmaceutical research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran mohammad ramezani nanotechnology research center, school of pharmacy, mashhad university of medical sciences, mashhad, iran

objective(s): first, 10-bromodecanoic acid was covalently attached to all available surface primary amines of ppi g2 and g3 to increase their lipophilicity. in the subsequent step, ppis were conjugated to the alkylcarboxylate groups of alkylcarboxylate-ppi derivatives to increase the number of surface primary amines. physicochemical properties of modified ppis were determined. transfection expe...

Objective(s): First, 10-bromodecanoic acid was covalently attached to all available surface primary amines of PPI G2 and G3 to increase their lipophilicity. In the subsequent step, PPIs were conjugated to the alkylcarboxylate groups of alkylcarboxylate-PPI derivatives to increase the number of surface primary amines. Physicochemical properties of modified PPIs were determined. Transfection expe...

Hossein Naderi-Manesh, Salim Albukhaty, Taki Tiraihi,

Background: The magnetic nanoparticle-based transfection method is a relatively new technique for delivery of functional genes to target tissues. We aimed to evaluate the transfection efficiency of rat neural stem cell (NSC) using poly-L-lysine hydrobromide (PLL)-coated super paramagnetic iron oxide nanoparticles (SPION). Methods: The SPION was prepared and coated with PLL as transfection agent...

Ali Dehshahri Mohammad Ramezani, Reza K. Oskuee Wayne. T. Shier

Objective Polyethylenimine (PEI), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. But it has been reported that PEI is cytotoxic in many cell lines. In this study, in order to enhance the transfection efficiency of 10 kDa PEI and reduce its toxicity, hydrophobic residues were grafte...

Journal: : 2022

Background. Various cationic polymers have been proposed for use as carriers of genetic materials in biotechnology and biomedicine. The poly(2-dimethylamino)ethyl-methacrylate (poly(DMAEMA)) demonstrated high transfection efficiency low cytotoxicity when used a gene delivery system. aim the present study was to evaluate ability poly(DMAEMA)-block-poly(N-vinylpyrrolidone)-co-(butyl-acrylate)-co-...

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