our previous studies showed that the promyelocytic leukemia protein, pml, functions as acellular and growth suppressor. transient expression of pml was also found to repress the activity of theepidermal growth factor receptor (egfr) gene promoter. in this study, we have examined the effects ofpml on a431 cells, which express a high level of egfr protein. the pml gene was introduced into thecell...

Background: Photodynamic therapy (PDT) is a promising therapeutic modality for the treatment of cancer and other diseases. In this study, the epidermoid carcinoma cell line A431 and the normal fibroblasts were used to investigate whether gold nanoparticles (GNPs) can induce an increase in cell death during PDT using 5-aminolevulinic acid (5-ALA) as a photosensitizer.Methods: Human fibroblast an...

background: photodynamic therapy (pdt) is a promising therapeutic modality for the treatment of cancer and other diseases. in this study, the epidermoid carcinoma cell line a431 and the normal fibroblasts were used to investigate whether gold nanoparticles (gnps) can induce an increase in cell death during pdt using 5-aminolevulinic acid (5-ala) as a photosensitizer. methods: human fibroblast a...

Metastasis is a major cause of mortality in cancer patients. Invadopodia are considered to be crucial structures that allow cancer cells to penetrate across the extracellular matrix (ECM) by using matrix metalloproteinases (MMPs). Previously, we isolated a highly invasive A431-III subline from parental A431 cells by Boyden chamber assay. The A431-III cells possess higher invasive and migratory ...

Clostridium perfringens iota-toxin is a binary toxin composed of an enzyme component (Ia) and a binding component (Ib). Each component alone lacks toxic activity, but together they produce cytotoxic effects. We examined the cytotoxicity of iota-toxin Ib in eight cell lines. A431 and A549 cells were susceptible to Ib, but MDCK, Vero, CHO, Caco-2, HT-29, and DLD-1 cells were not. Ib bound and for...

Proliferation of some cultured human tumor cell lines bearing high numbers of epidermal growth factor (EGF) receptors is paradoxically inhibited by EGF in nanomolar concentrations. In the present study, we have investigated the biochemical mechanism of growth inhibition in A431 human squamous carcinoma cells exposed to exogenous EGF. In parallel, we studied a selected subpopulation, A431-F, whi...

Sanguinarine, derived from the root of Sanguinaria canadendid, has been shown to possess antimicrobial, anti-inflammatory, and antioxidant properties. Here we compared the antiproliferative and apoptotic potential of sanguinarine against human epidermoid carcinoma (A431) cells and normal human epidermal keratinocytes (NHEKs). Sanguinarine treatment was found to result in a dose-dependent decrea...

conclusions this study delineated the beneficial role of rsv for growth regulation of skin cancer cells and highlighted the involvement of rsv in the induction of apoptosis in a431 cells. these findings might conduct evidence into the oncolytic properties of rsv in the skin cancer. further studies are required to indicate intracellular targets for rsv-induced apoptosis in skin cancer cells. res...

Adenoviral vectors directing the expression of HB-EGF and ADAM 12S were used to better understand the gene expression profile of cellular reprogramming into BAT-like cells compared to HB-EGF and ADAM 12S transfection. Human epidermoid carcinoma cells (A431) were co-infected with HB-EGF and ADAM 12S adenoviruses, monitored for fluorescence and lipid accumulation for 1-3 weeks. Cells were stained...

Monoclonal antibodies (MoAbs) were raised against epidermal growth factor (EGF) receptors on a human epidermoid carcinoma cell line, A431. Administration of anti-EGF receptor MoAbs inhibited tumor formation in athymic mice by A431 cells and by another epidermal carcinoma cell line, T222. When one of the same MoAbs was used in therapy against Li-7 (a human hepatoma) and HeLa cells (a cervical ca...