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the aim of this study was to compare three routine precipitation methods, including: acetone, tca/acetone wash and tca/acetone. 30 plasma samples were precipitated using above mentioned three methods. pellets were dissolved in rehydration solution and protein quantification was done for this solution. according to statistical analysis using spss ver 16.0 software and one-way anova test, the pro...

The metabolism of acetone was investigated in the actinomycete Rhodococcus rhodochrous (formerly Nocardia corallina) B276. Suspensions of acetone- and isopropanol-grown R. rhodochrous readily metabolized acetone. In contrast, R. rhodochrous cells cultured with glucose as the carbon source lacked the ability to metabolize acetone at the onset of the assay but gained the ability to do so in a tim...

Exhaled acetone is measured to estimate exposure or monitor diabetes and congestive heart failure. Interpreting this measurement depends critically on where acetone exchanges in the lung. Health professionals assume exhaled acetone originates from alveolar gas exchange, but experimental data and theoretical predictions suggest that acetone comes predominantly from airway gas exchange. We measur...

Intraperitoneal injection of 5 mumol of acetone/g, body weight, into 3 rats previously fed 1% acetone (v/v) in their drinking water resulted in the appearance in blood serum of 16 +/- 2 nmol of 1,2-propanediol/ml and 8 +/- 1 nmol of 2,3-butanediol/ml. No detectable 1,2-propanediol or 2,3-butanediol was found in the serum of animals after acetone or saline injection without prior addition of ace...

A mixed culture, WoAct, growing on acetone, consisted of two dominant morphotypes: a rod-shaped acetone-fermenting bacterium producing acetate, and an acetate-utilizing Methanosaeta species. Dense cell suspensions, largely free of the aceticlastic methanogen and supplemented with bromoethanesulfonate, were able to degrade acetone and grow in small volumes in membrane-separated culture devices i...

In the determination of P-oxybutyric acid through oxidation to acetone by the Shaffer bichromate method’ with subsequent estimation of the acetone by Messinger’s iodimetric titration,” difficulty was experienced in obtaining the t,heoretical amount of acetone from known amounts of pure P-oxybutyric acid. It was suspected that either the Messinger titration was inaccurate or that the acetone was...