the field of vaccinology provides excellent promises to control different infectious and non-infectious diseases. genetic immunization as a new tool in this area by using naked dna has been shown to induce humoral as well as cellular immune responses with high efficiency. this demonstrates the enormous potential of this strategy for vaccination purposes. dna vaccines have been widely used to de...

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

introduction: immune system is involved in the etiology and pathophysiology of inflammation and vitamins are important sources of substances inducing nonspecific immunomodulatory effects. given the proinflammatory role of cytokines in the inflammation and pain induction, this study aimed to assess the effects of long-term administration of vitamin b1 on the proinflammatory cytokines, edema, and...

Background & Objective: DNA vaccines as a new generation of vaccines require adjuvant to improve vaccine immunogenicity; adjuvants can also increase the DNA vaccine efficacy. In this study, the effects of the host’s interferon-inducible Mx protein as bio adjuvant and conventional alum adjuvant were evaluated.   Materials & Methods: The BALB/c mice were immunized by different prime-boost strate...

we have previously shown the adjuvant activity of propranolol (prp) (a beta-adrenergic receptor antagonist) using a vaccine model for salmonella typhimurium. in this study prp was used as an adjuvant in combination with plasmodium  berghei  (p. berghei)  whole blood stage (pwbs) antigens. balb/c mice were immunized three times with a 2-week interval, either pwbs vaccine alone or in combination ...

background and objectives rheumatoid arthritis (ra) is a chronic inflammatory disease which is characterized by joint swelling, and synovial inflammation. c reactive protein (crp) and ceruloplasmin (cp) are identified as important biomarkers of ra and various inflammatory diseases. curcumin, a widely used yellow color spice is the most active component of curcuma longa l (turmeric). curcumin co...

background: adjuvants are used to increase the immunogenicity of new generation vaccines, especially those based on recombinant proteins. despite immunostimulatory properties, the use of bacterial lipopolysaccharide (lps) as an adjuvant has been hampered due to its toxicity and pyrogenicity. brucella abortus lps is less toxic and has no pyrogenic properties compared to lps from other gram negat...

background and objectives : haemorrhagic septicaemia (hs), caused by pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in india. oil adjuvant vaccine (oav) is the most potent vaccine available for the control of hs. the study aims to evaluate the effect of alum co-adjuvantation of oav on emulsion stability and immune response. materials and methods : two dif...

background: recent researches have led to find strategies to prevent relapse and to improve survival for gastric cancer patients, including preoperative neo-adjuvant approaches. however, the efficacy of some neo-adjuvant regimens including 5-fluorouracil, cisplatin, and docetaxel have been less investigated. the present study evaluated the outcome and mid-term survival of patients with gastric ...