Fungi colonizing senescent chickpea (Cicer arietinum) stems and postharvest debris from Pullman, WA, were enumerated and identified with the objective of finding species potentially useful for biological control of Didymella rabiei (conidial state 1⁄4 Ascochyta rabiei), causal agent of Ascochyta blight. In addition to D. rabiei, primary colonizers were, in order of decreasing abundance, Alterna...

Transcription factors (TFs) are the key players in gene expression and their study is highly significant for shedding light on the molecular mechanisms and evolutionary history of organisms. During host-pathogen interaction, extensive reprogramming of gene expression facilitated by TFs is likely to occur in both host and pathogen. To date, the knowledge about TF repertoire in filamentous fungi ...

The most important foliar diseases in legumes worldwide are ascochyta blights. Up to now, in the Ascochyta-legume pathosystem most studies focused on the identification of resistance genes in the host, while very little is known about the pathogenicity factors of the fungal pathogen. Moreover, available data were often obtained from fungi growing under artificial conditions. Therefore, in this ...

The historical and contemporary population genetic structure of the chickpea Ascochyta blight pathogen, Ascochyta rabiei (teleomorph: Didymella rabiei), was determined in the US Pacific Northwest (PNW) using 17 putative AFLP loci, four genetically characterized, sequence-tagged microsatellite loci (STMS) and the mating type locus (MAT). A single multilocus genotype of A. rabiei (MAT1-1) was det...

Cicer arietinum, Ascochyta rabiei, Elicitor. Isoflavones, Phytoalexins Upon slicing cotyledons of chickpea, Cicer arietinum L ., accumulate the pterocarpan phyto­ alexins medicarpin and maackiain. Treatment of this tissue with an elicitor from the phytopathogenic deuteromycete Ascochyta rabiei (Pass.) Lab. greatly enhances accumulation of the pterocarpans and of other isoflavones and flavonoids...

The aim of this study was to isolate, identify and characterize ascochyta blight pathogens from Cicer judaicum , a wild annual Cicer species which grows in Israel and other Mediterranean countries in sympatric distribution with legume crops, and determine their virulence and aggressiveness to other wild and domesticated legumes. Native C. judaicum plants exhibited symptoms resembling ascochyta ...

Ascochyta blight caused by Ascochyta rabiei (Pass.) Labrousse is a major biotic constraint in production of chickpea. In the present investigation, all chickpea genotypes [E100Y (m), Gaurav, Pb-7 and L550] induced 100% callus on standard medium with greenish colour and fragile structure. These calli were used for in vitro screening against pathogen, A. rabiei culture filtrate at 0, 5, 10, 15 an...

Crude protein extracts from the chickpea (Cicer arietinum L .) pathogenic fungus Ascochyta rabiei catalyze the N A D PH dependent conversion of the pterocarpan phytoalexins medicarpin and maackiain to 1) 2'-hydroxyisoflavans, 2) 1 a-hydroxypterocarp-l,4-diene-3-one, and 3) 10,2'dihydroxyisoflav-8-ene-7-one derivatives. A pterocarpan: N A D PH oxidoreductase which cleaves the benzylphenyl ether ...

The antimycotic potential of Datura metel (syn. Datura alba Nees.) was investigated in vitro against Ascochyta rabiei, the cause of chickpea blight disease. The pathogen was exposed to various n-hexane concentrations (1, 2, 3 and 4% w/v) of shoot and root extracts of D. metel using poisoning food technique. All the employed concentrations of both root and shoot extracts significantly suppressed...

Evolutionary relationships were inferred among a worldwide sample of Ascochyta fungi from wild and cultivated legume hosts based on phylogenetic analyses of DNA sequences from the ribosomal internal transcribed spacer regions (ITS), as well as portions of three protein-coding genes: glyceraldehyde-3-phosphate-dehydrogenase (G3PD), translation elongation factor 1-alpha (EF) and chitin synthase 1...