OBJECTIVE Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. METHODS A total of 112 fecal specimens were examined by conventional methods: Direct...

Hagemann (Muench. Med. Wochschr. 85:1066, 1938) discovered that tubercle bacilli have selective affinity for phenol-auramine stain. Since then, several investigators (Richards, Kline, and Leach, Am. Rev. Tuberc. 4:255, 1941; Bogen, Am. Rev. Tuberc. 4:267, 1941; Lempert, Lancet 2:818, 1944; Gray, Am. Rev. Tuberc. 68:82, 1953; Kuper and May, J. Pathol. Bacteriol. 79:59, 1960) have advocated its u...

AIMS Despite its long history, the acid fast smear remains unstandardised. Technical variations in both the preparation of clinical material and subsequent staining mean that smear sensitivity relative to culture may vary from 50% to over 80%. This study assessed the sensitivity of acid fast microscopy at each of five stages of sample preparation and by both commonly used staining methods. ME...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears ...

Introduction and Aim: Tuberculosis (TB) remains a major public health concern with its high incidence mortality. In developing countries, smear microscopy continues to be the primary diagnostic tool for diagnosis of TB. Sputum samples processing under resource-limited settings could hazardous, which urges safe efficient techniques. This study aimed evaluate efficacy two different pot methods: P...

Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between prepa...

The Ziehl-Neelsen stain is compared with three alternative methods of staining tubercle bacilli in paraffin sections: Fite's method (1938); a modification by Armstrong and Price (1947) of Fite's method; and a fluorescent method using the auramine-phenol stain. The fluorescent method was found to be the most effective of the four methods and its use is recommended as a routine tool in diagnostic...

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...