FlcA is a response regulator controlling flocculation and the morphological transformation of Azospirillum cells from vegetative to cyst-like forms. To understand the cellular responses of Azospirillum to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in A. brasilense Sp7 and its flcA knockout mutant were investigated. Compari...

Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifH probe derived from Azospirillum...

Flagellation of a nonswimming variant of the mixed flagellated bacterium Azospirillum lipoferum 4B was characterized by electron microscopy, and polyclonal antibodies were raised against polar and lateral flagellins. The variant cells lacked a polar flagellum due to a defect in flagellin synthesis and constitutively expressed lateral flagella. The variant cells were able to respond to condition...

the main objective of this study was to determine the effects of nitrogen fixing bacteria and manure application on the seed yield and yield components in black cumin (nigella sativa l.). the experiment was carried out at the ran research station in firouzkouh in 2012. a 4×4 factorial experiment, arranged in a randomized complete blocks designed with three replications. the treatments consisted...

BACKGROUND: Azospirillum is effective in biostabilization of nitrogen and Pseudomonas is effective in dissolving soil phosphorus. OBJECTIVES: Current study was conducted to assess effect of different level of nitrogen and biofertilizer on seed yield, its components and protein content. METHODS: This research was carried out via spilt plot fac...

A pot experiment was carried out during 2009 and 2010 to study the field performance of blue pine seedlings inoculated with selected species of bio-inoculants under nursery conditions .The experiment was laid in Completely Randomized Design with three replications which comprised of seven inoculants (Azotobacter sp., Azospirillum sp., Pseudomonas fluorescens, Bacillus subtilis, Pisolithus tinct...

The rhizosphere bacterium Azospirillum amazonense associates with plant roots to promote plant growth. Variation in replicon numbers and rearrangements is common among Azospirillum strains, and characterization of these naturally occurring differences can improve our understanding of genome evolution. We performed an in silico comparative genomic analysis to understand the genomic plasticity of...

Ammonium chloride (greater than or equal to 0.05 mM) effectively and reversibly inhibited the nitrogenase activity of Azospirillum brasilense, Azospirillum lipoferum and Azospirillum amazonense. The glutamine synthetase inhibitor L-methionine-DL- sulfoximine abolished this "switch-off" in A. lipoferum and A. brasilense, but not in A. amazonense. Azaserine, an inhibitor of glutamate synthase, in...

Inoculation of corn (Zea mays) seeds with Azospirillum brasilense strain Cd or Sp 7 significantly enhanced (30 to 50% over controls) the uptake of NO(3), K, and H(2)PO(4) into 3- to 4-day- and 2-week-old root segments. No gross changes in root morphology were observed; altered cell arrangement in the outer four or five layers of the cortex was seen in photomicrographs of cross sections of inocu...