context advanced glycation end-products (ages) are signaling proteins associated to several vascular and neurological complications in diabetic and non-diabetic patients. ages proved to be a marker of negative outcome in both diabetes management and surgical procedures in these patients. the reported role of ages prompted the development of pharmacological inhibitors of their effects, giving ri...

Accumulation of triosephosphates arising from high cytosolic glucose concentrations in hyperglycemia is the trigger for biochemical dysfunction leading to the development of diabetic nephropathy-a common complication of diabetes associated with a high risk of cardiovascular disease and mortality. Here we report that stimulation of the reductive pentosephosphate pathway by high-dose therapy with...

Results: Endothelial cells exhibited high viability (>90%) as measured via confocal microscopy using Hoechst 33342 and propidium iodide staining. Following 7 days of incubation, the necessary structural integrity of the endothelial monolayer lining the lumen was achieved. This was proven by the formation of a well-defined network of actinfilaments, stained with Alexa Fluor 488 ̈ phalloidin. Addi...

AIM Efficacy and safety of benfotiamine in treatment of diabetic polyneuropathy. METHODS Double blind, placebo-controlled, phase-III-study. 181 patients were screened. 165 patients with symmetrical, distal diabetic polyneuropathy were randomised to one of three treatment groups entering the wash-out phase and 133/124 patients were analysed in the ITT/PP analysis: Benfotiamine 600 mg per day (...

We designed a transdermal system to serve as a delivery platform for benfotiamine utilizing the attributes of passive penetration enhancing molecules to penetrate through the outer layers of skin combined with the advance of incorporating various peripherally-acting vasodilators to enhance drug uptake. Benfotiamine, incorporated into this transdermal formulation, was applied to skin in an anima...

Benfotiamine has shown therapeutic efficacy in the treatment of painful diabetic neuropathy in human beings. However, so far there is no evidence about the efficacy of this drug in preclinical models of pain. The purpose of this study was to assess the possible antinociceptive and antiallodynic effect of benfotiamine in inflammatory and neuropathic pain models in the rat. Inflammatory pain was ...

Chronic microglial activation and resulting sustained neuroinflammatory reaction are generally associated with neurodegeneration. Activated microglia acquires proinflammatory cellular profile that generates oxidative burst. Their persistent activation exacerbates inflammation, which damages healthy neurons via cytotoxic mediators, such as superoxide radical anion and nitric oxide. In our recent...

Adiponectin regulates insulin sensitivity (1), reduces the expression of endothelial adhesion molecules (2), and has anti-inflammatory effects (3). Decreased adiponectin levels accompany obesity (4) and type 2 diabetes (5), promoting insulin resistance (5) and cardiovascular disease (6,7). Data on postprandial adiponectin regulation in different populations are controversial, with studies showi...