Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...

background: stress may cause damages to dna or/and change the ability of the cells to overcome these damages. it may also cause irregularities in the cell cycle and induce abnormal cell divisions through glucocorticoid-dependent functions. the abnormal cell divisions, in turn, lead to chromosomal mal-segregation and aneuploidy. in this study, the effects of the stress hormone, hydrocortisone (h...

Purpose: Binucleation is a reactive cellular change (RCC) in Pap smears due to Candida infection. However, the origin of these binucleated cells as RCCs remains unclear. The purpose of this study was to examine binucleation in patients negative for intraepithelial lesion or malignancy (NILM) and infected with Candida and those infected with high-risk human papillomavirus (hr-HPV) and to clarify...

Fusion of bone marrow-derived cells with adult Purkinje cells in the cerebellum gives rise to binucleated Purkinje cells. Whether fusion can be modulated by epigenetic factors and whether fused neurons are stable has remained unclear. Here, we show that in mice and rats, partial ablation of Purkinje cells and local microglial activation in the absence of structural damage to the cerebellum incr...

INUCLEATED cells occur in a variety of tissues in plants and animals normally and under varying physiological conditions (e.g., anemia, stress, ageing, regeneration, malignancy and viral infection) (BLOOM 1968). Among animals, naturally occurring binucleated cells have been observed in organisms of the following classes: Protozoa, Nematoda, Crustacea, Arachnida, Insecta, Agnatha, Pisces, Amphib...

The mammary gland represents a unique tissue to study organogenesis as it predominantly develops in the post-natal animal and undergoes dramatic morphogenetic changes during puberty and the reproductive cycle. The physiological function of the mammary gland is to produce milk to sustain the newborn. Here we view the lactating gland through three-dimensional confocal imaging of intact tissue. We...

Background: Most of the cytogene c dosimetry, except Premature Chromosome Condensa on assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB‐MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0‐15Gy, cultured for 72 h. Cytochal...

OBJECTIVE A micronucleus is a small additional nucleus formed due to chromosomal loss or fragmentation. The aim of this study was to assess the utility of micronucleus count as a screening tool in cervical cancer. MATERIAL AND METHOD A total of 132 cases consisting of 42 pre-neoplastic and neoplastic cases and 90 non-neoplastic cases were studied. Only routine Papanicolaou-stained cervical sm...

The postnatal proliferative activity of retinal pigment epithelium (RPE) cells and its dependence on intraocular pressure were studied using [3H]thymidine and [14C]thymidine autoradiography in normal and experimentally induced microphthalmic pigmented rats. The regulation of RPE growth by intraocular pressure was shown to involve the control of the number of binucleated cells by means of stimul...