Wastewater discharged from clinical isolation and general wards at two hospitals in Singapore was examined to determine the emerging trends of antibiotic resistance (AR). We quantified the concentrations of 12 antibiotic compounds by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS), antibiotic-resistant bacteria (ARB), the class 1 integrase gene (intI1), and 16 antibioti...

To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (bl...

Escherichia coli TOP10. Plasmid analysis of the K. pneumoniae isolate and its transformant confirmed that blaKPC-2 was located on an 75 kb plasmid. No other antibiotic resistance marker was co-transferred. PCR-based replicon typing of the major plasmid incompatibility groups showed that the blaKPC-2positive plasmid belonged to the IncFIIAS incompatibility group. We believe this is the first rep...

Aim Carbapenems are antibiotics reserved for treatment of severe infections. Carbapenem antimicrobial susceptibility testing profiles were determined in a population of Klebsiella pneumoniae, and their resistance assessed based on previous and current Clinical and Laboratory Standards Institute criteria. Materials & methods Isolates were examined using an automated antimicrobial susceptibilit...

The presence of pan-resistant bacteria worldwide possesses a threat to global health. It is difficult to evaluate the extent of carriage of resistant bacteria in the population. Sewage sampling is a possible way to monitor populations. We evaluated the presence of pan-resistant bacteria in Israeli sewage collected from all over Israel, by modifying the pour plate method for heterotrophic plate ...

Klebsiella pneumoniae is an important nosocomial pathogen, and multidrug-resistant strains have become a worldwide concern. Here, we report the complete genome of a K. pneumoniae isolate with chromosomally integrated blaKPC genes and a colibactin synthesis locus.

Sir, The production of carbapenem-hydrolysing b-lactamases is increasingly reported in Enterobacteriaceae. Among the different types of carbapenemases, the emergence of the Ambler class A KPC-type b-lactamases is of great concern, since those enzymes hydrolyse all b-lactams with the exception of cephamycins. Enterobacterial isolates producing KPC-type b-lactamases were reported in many areas in...

Wildlife may harbour clinically important antimicrobial-resistant bacteria, but the role of wildlife in epidemiology bacterial infections humans is largely unknown. In this study, we aimed to assess dissemination blaKPC carbapenemase gene among and gulls Alaska. We performed whole-genome sequencing determine genetic context isolates from all four human carbapenemase-producing Enterobacteriaceae...

We characterized Escherichia coli ST131 isolates among 116 carbapenemase-producing strains. Of isolates from 16 countries collected during 2008-2013, 35% belonged to ST131 and were associated with blaKPC, H30 lineage, and virotype C. This study documents worldwide incidents of resistance to "last resort" antimicrobial drugs among a common pathogen in a successful sequence type.

OBJECTIVE Enterobacteriaceae bacteria harboring Klebsiella pneumoniae carbapenemase are a serious worldwide threat. The molecular identification of these pathogens is not routine in Brazilian hospitals, and a rapid phenotypic screening test is desirable. This study aims to evaluate the modified Hodge test as a phenotypic screening test for Klebsiella pneumoniae carbapenemase. METHOD From Apri...