نتایج جستجو برای: brucella melitensis
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INTRODUCTION Brucella melitensis is a facultative intracellular Gram-negative bacterial pathogen that may enter the host via ingestion or inhalation, or through conjunctiva or skin abrasions. Some Brucella spp surface proteins (SPs) play an important role in bacterial adhesion and invasion and thus represent targets for the host immune system. Brucella spp surface protein with apparent molecula...
BACKGROUND The Qinghai-Tibet Plateau (QTP) of China is an extensive pastoral and semi-pastoral area, and because of poverty and bad hygiene conditions, Brucella is highly prevalent in this region. In order to adequately prevent this disease in the QTP region it is important to determine the identity of Brucella species that caused the infection. METHODS A total of 65 Brucella isolates were ob...
Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes...
Brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the VirB type IV secretion system and the flagellar genes. However, the basis of quorum sensing, namely the production of autoinducers in Brucella has been questioned. Here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-c...
The incidence of human brucellosis in Kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. The latest species identification of Brucella isolates in Kyrgyzstan was carried out in the 1960s and investigated the circulation of Brucella abortus, B. melitensis, B. ovis, and B. suis. However, suppo...
Background and purpose: Brucellosis is a zoonotic disease. Given that serologic test has some specific characteristics such as difficult growth, problems associated with culture, and cross-reactions with otherbacteria, in this study multiplex real-time polymerase chain reaction (PCR) method was used to detect the most common species of Brucella spp. Materials and methods: This experimental s...
WORTHINGTON, R. W. & MULDERS, MARIAS. G. Antigenic relationship of Brucellaovis to Brucella abortus and Brucella melitensis using the complement fixation test. Onderstepoort J. vet. Res., 36 (2) , 191-1 98, 1969. The CF test was used to investigate the serological relationship of Br. avis to Br. abortus and Br. melitensis. A definite antigenic relationship between Br. avis and Br. abortus could...
Immune sera were prepared in rabbits by the injection of living and acetone-killed cells of Brucella ovis and smooth and rough B. melitensis. The use of whole-cell antigens in agglutination and agglutinin-absorption tests revealed little relationship between B. ovis and smooth B. melitensis, although there was extensive cross-agglutination between B. ovis and rough B. melitensis. The use of wat...
We read with interest the paper “Ribosomal RNA Sequence Analysis of Brucella Infection Misidentified as Ochrobactrum anthropi Infection” by Horvat and colleagues (1). The paper reported that a Brucella isolate was misidentified as Ochrobactrum anthropi. The 16S rRNA results indicated that the isolates were Brucella species, with 100% agreement to rRNA sequences of known brucellae and low homolo...
Background: The intrinsic heterogeneity determination in Brucella Lipopolysaccharide (LPS) is important for explaining its chemicalnature and biological behavior. This is significant for practical purposes, since LPS is the most relevant antigen during infectionand vaccination.Objectives: The purpose of the present study was to compare biochemical and immunological differences of LPS and lipid ...
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