نتایج جستجو برای: cell seeding density

تعداد نتایج: 2049618  

In vitro studies on cancer cell lines still constitutes a major part of cancer research. It is very important that the researchers consider the importance of basic conditions of working with different cell lines. To the best of authors knowledge, this study is the first one to report the effect of seeding density on the growth curve of three human cancer cell lines. These cell lines include hum...

Journal: :iranian journal of basic medical sciences 0
mohamadreza baghaban eslaminejad stem cell department, cell science research centre, royan institute, acecr, tehran, iran hamid nazarian stem cell department, cell science research centre, royan institute, acecr, tehran, iran fahimeh falahi stem cell department, cell science research centre, royan institute, acecr, tehran, iran leila taghiyar stem cell department, cell science research centre, royan institute, acecr, tehran, iran mohamad taghi daneshzadeh royan animal facility, karaj, iran

objective(s) mesenchymal stem cells (mscs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. the present study tried to isolate and characterize mscs from goat bone marrow. materials and methods fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. passaged-3 cells were then differentiat...

Journal: :avicenna journal of medical biotechnology 0

background: despite major progress in our general knowledge related to the application of adult stem cells, finding alternative sources for bone marrow mesenchymal stem cells (mscs) has remained to be challenged. in this study successful isolation, multilineage differentiation, and proliferation potentials of sheep mscs derived from bone marrow, adipose tissue, and liver were widely investigate...

2013
Fei Luo Tian-Yong Hou Ze-Hua Zhang Zhao Xie Xue-Hui Wu Jian-Zhong Xu

This study aimed to study the effects of initial cell density and in vitro culture method on the construction of tissue-engineered bone grafts and osteogenic activities. Human mesenchymal stem cells (hMSCs) were seeded onto cubic scaffolds prepared from demineralized bone matrix (DBM) by three methods - static, hydrodynamic, or fibrin hydrogel-assisted seeding. The resulting cell-scaffold const...

2012
Jeroen Eyckmans Grace L. Lin Christopher S. Chen

It has previously been demonstrated that cell shape can influence commitment of human bone marrow-derived mesenchymal stem cells (hBMCs) to adipogenic, osteogenic, chondrogenic, and other lineages. Human periosteum-derived cells (hPDCs) exhibit multipotency similar to hBMCs, but hPDCs may offer enhanced potential for osteogenesis and chondrogenesis given their apparent endogenous role in bone a...

Journal: :Artificial organs 2008
Ravi Birla Vikas Dhawan Yen-Chih Huang Ian Lytle Khajohn Tiranathanagul David Brown

Experiments have been successfully performed culminating in functional, vascularized, three-dimensional cardiac muscle tissue. Past experience in tissue engineering has led us to the understanding that cell seeding density plays a critical role in the formation and function of both in vitro and in vivo engineered tissues. Therefore, to improve upon the mechanics of this model and to facilitate ...

Journal: :Journal of biomedical materials research 2000
C E Holy M S Shoichet J E Davies

New three-dimensional (3D) scaffolds for bone tissue engineering have been developed throughout which bone cells grow, differentiate, and produce mineralized matrix. In this study, the percentage of cells anchoring to our polymer scaffolds as a function of initial cell seeding density was established; we then investigated bone tissue formation throughout our scaffolds as a function of initial c...

Journal: :Journal of biomedical materials research 1998
J Gao L Niklason R Langer

A procedure for surface hydrolysis of poly(glycolic acid) (PGA) meshes was developed to increase cell seeding density and improve attachment of vascular smooth muscle cells. Hydrolysis of PGA in 1N NaOH transformed ester groups on the surface of PGA fibers to carboxylic acid and hydroxyl groups. After hydrolysis, the polymer scaffold retained its original gross appearance and dimensions while t...

Journal: :Journal of biomedical materials research. Part A 2015
Mohammed A Yassin Knut N Leknes Torbjorn O Pedersen Zhe Xing Yang Sun Stein A Lie Anna Finne-Wistrand Kamal Mustafa

Constructs intended for bone tissue engineering (TE) are influenced by the initial cell seeding density. Therefore, the objective of this study was to determine the effect of bone marrow stromal stem cells (BMSCs) density loaded onto copolymer scaffolds on bone regeneration. BMSCs were harvested from rat's bone marrow and cultured in media with or without osteogenic supplements. Cells were seed...

2012
E. Fossett W. S. Khan

Adult mesenchymal stem cells (MSCs) are being investigated further for their use in stem cell therapies. However, as they are found in very low numbers in adult tissue, expansion in vitro is required to produce desired MSC numbers for clinical application. The need for effective cell-based therapies is increasing due to a rise in the ageing population, increasing the prevalence of musculoskelet...

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