نتایج جستجو برای: cell seeding density

تعداد نتایج: 2049618  

2017
Divya B Patel Kelsey M Gray Yasasvhinie Santharam Tek N Lamichhane Kimberly M Stroka Steven M Jay

Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have emerged as potential therapeutic agents for numerous applications. EVs offer potential advantages over cell-based therapies with regard to safety, stability and clearance profiles, however production and potency limitations must be addressed to enable eventual translation of EV-based approaches. Thus, we sought to examine the...

Journal: :Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia 2011
Hiroki Yokomuro Noritsugu Shiono Yoshinori Watanabe Katsunori Yoshihara Nobuya Koyama Mitsumasa Okada

BACKGROUND Tissue engineering with cell seeded biodegradable material has attracted attention as a novel means of treating the severely impaired heart. Here, we consider optimal preparation of a durable biograft using dynamic and static cultures. METHODS Vascular smooth muscle cells (VSMCs) derived from the rat aorta were seeded onto biodegradable material P (LA/CL) (poly-L-lactide-ε-caprolac...

Journal: :international journal of hematology-oncology and stem cell research 0
faten abo-aziza mohamed abo-aziza department of parasitology and animal diseases, veterinary research division, national research center, giza, egypt abd el-kader ahmed zaki department of physiology, college of veterinary medicine, cairo university, giza, egypt

background: in the field of cellular therapy, the impact of confluence degree on harvesting or differentiation of bmmscs and the effect of cell-to-cell contact remain controversial. therefore, the effect of confluence on properties of bmmscs was studied and efficiency of confluence-associated osteogenic differentiation was identified. materials and methods: the impact of 20, 50, 70, 80 and 100%...

Journal: :European cells & materials 2011
Simon Stephan W Eustace Johnson Sally Roberts

The adult human intervertebral disc (IVD) is normally avascular. Changes to the extracellular matrix in degenerative disc disease may promote vascularisation and subsequently alter cell nutrition and disc homeostasis. This study examines the influence of cell density and the presence of glucose and serum on the proliferation and survival of IVD cells in 3D culture. Bovine nucleus pulposus (NP) ...

Journal: :The British journal of surgery 1991
D A Mosquera M Goldman

Endothelial cell seeding is a technique that has developed over the past 15 years in response to the need for a high performance synthetic vascular graft. This review details our present knowledge of seeding and examines the various problems that have hampered its introduction into clinical practice.

2010

There is interest in the identification of the best seeding density for new corn hybrids and on reduced use of herbicides for weed control. The objective of this study was to evaluate the effects of seeding density (30, 50, 70, and 90 thousand plants ha) and weed control on green ear yield and grain yield in corn cultivar AG 1051. A completely randomized block design was adopted with split-plot...

ژورنال: علوم زراعی ایران 2020

To evaluate the effects of tillage and planting systems on soil physical properties, grain yied and its components, and milling recovery of rice (cv. Hashemi), an experiment was conducted at Rice Research Institute of Iran in 2017 and 2018 cropping seasons-using split plot arrangements in randomized complete block design with three replications. The experimental factors were; tillage methods (m...

Journal: :modares journal of medical sciences: pathobiology 2014
hoda jahandar vahid khalaj leila nematollahi fatemeh zandi somayeh enayati

objective: the study of physiological changes in recombinant cell lines provides useful information to improve production performance. in this study, we investigate the effects of an anti-cd33 chimeric igg4 expression on sp2.0 cell growth. methods: variable region genes of light and heavy chains of monoclonal antibody produced by m195 were cloned in pfuse-clig-hk and pfuse-chig-hg4 expression ...

Journal: :Biomaterials 2006
Stephanie M Willerth Kelly J Arendas David I Gottlieb Shelly Elese Sakiyama-Elbert

The objective of this research was to determine the appropriate cell culture conditions for embryonic stem (ES) cell proliferation and differentiation in fibrin scaffolds by examining cell seeding density, location, and the optimal concentrations of fibrinogen, thrombin, and aprotinin (protease inhibitor). Mouse ES cells were induced to become neural progenitors by adding retinoic acid for 4 da...

2011
Gary Adams Lee Buttery Stephen E. Harding Nan Wang

Mouse embryonic stem (ES) cells are continuous cell lines derived directly from the fetal founder tissue of the pre-implantation embryo and can be expanded in vitro and give rise to cells from ectodermal, mesodermal and endodermal layers. Mouse ES cells can be maintained and their numbers expanded by culture on feeder layer cells with LIF present in the culture medium. This study shows that cha...

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