Dynamical systems are often used to model biochemical and biological processes. In Seo et al. (2010, 2014) we studied two mathematical models of the iterative biochemical procedure known as SELEX (Systematic Evolution of Ligands by EXponential Enrichment): multiple target SELEX and alternate SELEX. It is the purpose of this paper to revisit the mathematics of these processes in the language of ...

Aptamers are nucleic acids referred to as chemical antibodies as they bind to their specific targets with high affinity and selectivity. They are selected via an iterative process known as 'selective evolution of ligands by exponential enrichment' (SELEX). Aptamers have been developed against numerous cancer targets and among them, many tumor cell-membrane protein biomarkers. The identification...

We give an analysis of the experimental material presented by the SELEX Collaboration to find the true place for the SELEX state D sJ(2632) in the unified picture for hadron spectra developed early. It is found that the SELEX measurements are excellently incorporated in the unified picture for hadron spectra. Our analysis shows that the measured values for the masses of the SELEX state exactly ...

BACKGROUND SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences binding a ligand of interest, called genomic aptamers. Concerns have been raised regarding requirements imposed on RNA sequences undergoing SELEX se...

The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candida...

The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols. In addition to simplifying the separation of binding and non-binding aptamer candidates, microbeads have facilitated the integration of other technologies such as emulsion PCR (ePCR) and Fluorescence Activated Cell Sorting (FACS) to high-throu...

Title of Document: CHARACTERIZATION OF NOVEL NUCLEIC ACID INHIBITORS (APTAMERS) OF HUMAN IMMUNODEFICIENCY VIRUS REVERSE TRANSCRIPTASE SELECTED USING A PRIMER-FREE SELEX APPROACH Yi-Tak Lai, Doctor of Philosophy, 2011 Directed By: Dr. Jeffrey J. DeStefano, Professor Department of Cell Biology and Molecular Genetics Aptamers are synthetic, single stranded nucleic acids that bind specifically to t...

Thrombin facilitates the aggregation of platelet in hemostatic processes and participates regulation cell signaling. Therefore, development thrombin sensors is conducive to comprehending role course a disease. Biosensors based on aptamers screened by SELEX have exhibited superiority for detection. In this review, we summarized aptamer-based detection which rely specific recognitions between apt...

The hope of success of therapeutic interventions largely relies on the possibility to distinguish between even close tumor types with high accuracy. Indeed, in the last ten years a major challenge to predict the responsiveness to a given therapeutic plan has been the identification of tumor specific signatures, with the aim to reduce the frequency of unwanted side effects on oncologic patients ...

Objective(s): Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressi...