the purpose of this investigation was to determine the antioxidant potentials and anti-glucosidase activities of six tropical medicinal plants. the levels of phenolic constituents in these medicinal plants were also quantified and compared. antioxidation potentials were determined colorimetrically for scavenging activities against dpph and no radicals. metal chelating assay was based on the mea...

A 5' nuclease assay has been developed to detect viable Listeria monocytogenes. The assay targets the hemolysin A (hlyA) transcript, which is found only in L. monocytogenes. The single-tube, reverse transcriptase (RT), fluorogenic probe-based assay was formatted by using Tth DNA polymerase whose activity was modulated by using the manganese-chelating morpholinepropanesulfonic acid (MOPS) buffer...

Chelating resins have been considered to be suitable materials for the recovery of heavy metals in water treatments. A chelating resin based on modified poly(styrene-alt-maleic anhydride) with Melamine was synthesized. This modified resin was further reacted with 1,2-diaminoethan in the presence of ultrasonic irradiation for the preparation of a tridimensional chelating resin on the nanoscale f...

Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Amo...

Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had highest antioxidant capacity. Four novel RP-HPLC UPLC-MS/MS. PGMLGGSPPGLLGGSPP SDGSNIHFPN DPPH radical scavenging activity (IC50 = 1.39 mM) Fe2+ chelating ability 4.60 mM), respec...

Objective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast adenocarcinoma) DLD-1 (colorectal cell lines. Methods: This study examined (25-200 µM) via DPPH, metal chelating reduction methods. Furthermore activity MCF-7, MCF-12A (normal epithelial) DLD-1, CCD-18CO colon fibroblast) were with MTT (3-(4,5-Dimethylthiazol-2-y...

A classic method for the encapsulation and culture of chondrocytes in alginate beads is described. Chondrocytes are released from cartilage matrix by collagenase/dispase digestion and mixed with a solution of 1.25% alginic acid until a homogenous suspension is obtained. The suspension is drawn into a syringe and pushed gently through a needle, so that drops fall into a solution of calcium chlor...

Barium sulfate (barite) is one of the widely used weighting materials in the preparation of drilling fluid for deep oil and gas wells. Barite is not soluble in the regular solvents; such as, hydrochloric acid (HCl) and other acids. Therefore, in this study, we focused on evaluating the dissolution of the industrial barite particles in different chelating agents. Chelating agents; such as, dieth...

The purpose of the study is to evaluate the relationship between the extraction parameters and the metal chelating activity of Centella asiatica (CA) and Erythroxylum cuneatum (EC). The response surface methodology was used to optimize the extraction parameters of methanolic extract of CA and EC with respect to the metal chelating activity. For CA, Run 17 gave optimum chelating activity with IC...