The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and pro...

isotherms of the binding of the anthracyciine antibiotic, adriamycin (adriblastin), to dna histone complexes was studied by means of spectroscopic analysis. the results indicated that: (a) binding of adriamycin to histones reduced the interaction of histones with dna, (b) binding of the drug to dna did not change the binding affinity of histone to dna and, (c) in the explored binding range of r

The fine structure of the cone and rod outer segments of the toad was studied under the electron microscope after fixation in osmium tetroxide and fixation in formaldehyde followed by chromation. In the OsO(4)-fixed specimens, the rod outer segment appears to be built of a stack of lobulated flattened sacs, each of which is made of two membranes of about 40 A separated by an innerspace of about...

NiCr alloys with different components were obtained by pack chromation and homogenization heat treatment of octet Ni lattice. The microstructure, alloy composition, microhardness quasi-static compression properties the lattice tested. results showed that after treatment, had an austenitic structure uniform composition. Compared pure nickel lattice, microhardness, compressive strength, elastic m...

Research on sex chromosome aberrations has made considerable progress. There are evidences that possession of an extra X chromosome may affect the mental health of an individual. All the male schizophrenia patients registered during the period of study, who were not under treatment, constituted the patient sample. They numbered two hundered eighty seven. A properly matched control sample of two...

The competence of the duodenal 1ƒ¿, 25-(OH)2-D3 receptor system in embryonic chicks was studied in the reconstituted cytosol-chro matin system. The sucrose density gradient centrifugation following incubation of duodenal cytosol with 1x,25-dihydroxy-[3H]-vitamin D3 revealed that a cytosol 1x,25-dihydroxyvitamin D3 receptor existed in the duodenum of 18-day-old embryos. In the duodenum of 14-day...

PURPOSE To obtain and analyze early retinal changes at the molecular level 24 h after a radiation injury to the ipsilateral intraorbital nerve using gamma knife surgery (GKS), and to examine the morphological changes in bilateral optic nerves. METHODS Unilateral intraorbital optic nerves of three rhesus macaques were treated by GKS with irradiated doses of 15 Gy, while contralateral optic ner...

Genetic engineering enables the construction of gene man Genome Project (5), efficient and robust production and purification strategies are necessary (6). For fusions resulting in fusion proteins having the combined properties of the original gene products. Fusion industrial production of recombinant proteins, simple and fast purification methods introduced as an early can be made on either or...