in this study, the chemical factors effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors on the activity of trypsin and chymotrypsin of rainbow trout fry were considered. the results showed that k+ and na+ had not significant decrease on trypsin and chymotrypsin activity than did those from control (p>0.05). ca2+ and mg2+ showed a significant increase in activity of trypsin ...

multiple sclerosis (ms) and its animal model, experimental autoimmune encephalomyelitis (eae), are inflammatory autoimmune diseases of the central nervous system. chymotrypsin is a serine protease with immunomodulatory effect in the peripheral organs. we previously demonstrated the immunomodulatory effect of chymotrypsin in ameliorating the eae in female lewis rats. however, there are sex-based...

Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T(2) phage is slowly inactivated by high concentrations of (alpha-, beta-, gamma-, or Delta-chymotrypsin, but not by trypsin or ficin. P(1) phage is slowly inactivated by alpha-, beta-, or gamma-chymotrypsin, or ficin, more rapidly by Delta-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystallin...

7-Amino-4-methylcoumarin (AMC) is a low molecular weight fluorescent probe that can be attached to peptide enable the detection of specific proteases, such as chymotrypsin, expressed in certain diseases. Because this depends on specificity protease toward peptidyl AMC, development substrates required. To investigate AMC compounds incorporating four different amino acid residues were prepared by...

PURPOSE Increase in intracellular chymotrypsin activity was reported during acute pancreatitis. Beside chymotrypsin, there are at least two enzymes with chymotrypsin-like activity: proteasome and lysosomal cathepsin A. Until now it is not known whether and to what extent they contribute to increases in chymotrypsin activity in acute pancreatitis. Our aim was to study organ chymotrypsin-like act...

Protein hydrolysate is highly regarded as a source of naturally occurring antioxidant peptides. The purpose of this study was to investigate the effect of Ultrasonic (Frequency, 20 KHz; Amplitude, 50%; Time, 30 min) and high-pressure homogenization (Power, 1500 bar; Rated flow, 10 dm/h) pretreatmenton the enzymatic hydrolysis and antioxidant properties of yeast protein hydrolysate obtained from...

A cDNA for a midgut chymotrypsin, induced by a blood meal, has been cloned and sequenced from the mosquito Aedes aegypti. The 938 base sequence codes for a 268 amino acid protein, which contains an 18-residue signal peptide and a seven-residue activation peptide. The deduced amino acid sequence contains several features typical of chymotrypsin proteases, including the catalytic triad of serine ...

The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency food transformation and growth, therefore aquaculture profitability. aim present was assess activity functional characteristics key enzymes in juveniles greater amberjack ( Seriola dumerili ), as well possible modulation their relative importance by water temperature. For that, a combinati...

Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through hydrolysis reaction, making it interesting subject to study activity of proteases. In this work, we compared detection chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods determined limit (LOD), 0.15 ± 0.01 nM for spectropho...