in this study, the possible infection of thomson navel, moro blood sweet orange, page mandarin and webber pomelo with citrus viroids was investigated. biological indexing was performed by grafting on etrog citron (c. medica) and maintained in temperature-controlled greenhouse. after inoculation, rna was extracted by sds– potassium acetate method. detection of citrus viroids was done reverse tra...

hop stunt viroid (hsvd) isolates have been reported as the causal agent of citrus cachexia in mazandaran province and recently shown to be associated with yellow corky vein disease of sweet orange and split bark disorder of sweet lime in the fars province, iran. in the present work isolation and partial characterization of viroids from citrus trees affected by gummy stem blight is reported from...

The Sudanese citrus industry is mainly based on oldline cultivars of grapefruit, sweet orange and willow leaf mandarin, grafted almost exclusively on sour orange. Infection with graft-transmissible agents, viroids in particular, may have deleterious effects on the productivity of these citrus species and, more importantly, limits the choice of rootstocks should sour orange be replaced by altern...

Background and Aims: CBLVd has been reported from northern of Iran previously. The aim of this study was evaluating new viroid variant from asymptomatic citrus trees of Fars province and comparing with its other isolates. Materials and Methods: In this study a number of citrus trees without symptoms were sampled and subjected to RNA extraction followed by RT-PCR, cloning and sequencing of ...

Citrus Bent Leaf Viroid (CBLVd) and Dwarfing (CDVd), from genus Apscaviroid, are two of the widely distributed citrus viroids among eight reported to date. They have been found induce reduction in canopy volume fruit production trees on trifoliate orange hybrids rootstocks. Moreover, infected only with CBLVd or combinations Exocortis (CEVd), Hop Stunt (HSVd) CDVd associated a poor development r...

gummy bark disease of sweet orange (citrus sinensis) trees has been reported from a few citrus growing regions of the world. the disease symptoms consist of mild to severe stem pitting and brown gummy flecks in the bark of sweet orange trees on sour orange ( c. aurantium) rootstock, being conspicuous several centimeter above the bud :union: on the trunk. the pits were usually impregnated with b...

citrus is one of the most important fruit crops in iran. among the citriculture areas of iran, mazandaran province has the highest acreage (about 100,000 ha), production and number of varieties nationwide. unauthorized importation of citrus species and cultivars and propagation by growers and nurserymen have led to perpetuation and spread of virus, viroid and other graft-transmissible pathogens...