BACKGROUND Identification of Staphylococci to species level in veterinary microbiology is important to inform therapeutic intervention and management. We report on the efficacy of three routinely used commercial phenotypic methods for staphylococcal species identification, namely API Staph 32 (bioMérieux), RapID (Remel) and Staph-Zym (Rosco Diagnostica) compared to genotyping as a reference met...

Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood specimens, including one pathogen and 5 contaminants and catheter colonizers. Bruck...

conclusions the cefoxitin disk diffusion method showed the best results when compared to oxacillin disk, similar to results from detecting the meca gene in pcr as a golden test. studies on mrsa that carry the luk-pv gene should continue to provide significant insight into the prevalence and epidemiology of these important resistant pathogens. also, the rate of pvl gene-producing isolates showed...

A total of 288 staphylococcal specimens isolated from different clinical specimens were selected for the evaluation of tests used to detect Staphylococcus aureus. The coagulase (coa) gene PCR was preformed, which confirmed 288 specimens as S. aureus and 51 specimens as coagulase negative staphylococci (CONS). All the specimens were subjected to slide coagulase test, Slidex Staph plus test and t...

Coagulase negative staphylococci have become prevalent nosocomial pathogens following the implantation of foreign bodies such as prosthetic valves, cerebrospinal fluid shunts, joint prostheses and central venous catheters. They are also the most common contaminants of blood cultures so that a means of differentiating contamination from a true bacteraemia is important.' Assessment of the clinica...

In 1954 we (Price and Kneeland) described a mucoid form of Micrococcus pyogenes var. aureus (Staphylococcus aureus) which showed capsular swelling with homologous immune serum. This mucoid variant, designated RL-mucoid, was derived from the parent RL strain after passage through embryonated eggs with influenza virus. Attempts to reproduce this variation by passing RL through embryonated eggs on...

Staphylococcus intermedius, a coagulase-positive staphylococcal species, is a common canine pathogen and a rare human wound pathogen. A total of 145 strains of S. intermedius (ATCC 29663, 4 reference strains, 4 human isolates, 44 canine infection isolates, and 92 isolates from canine gingiva) were screened for lysogenic phage by a modified Fisk method. Nineteen phage preparations were prepared ...

To test the hypothesis that the strain relatedness of coagulase-negative staphylococci (CoNS) recovered from blood cultures can be inferred from automated antibiotic susceptibility testing (AST) results generated by Vitek 2, concordant or discordant AST results were compared with pulsed-field gel electrophoresis (PFGE) typing results for 119 CoNS blood culture isolate pairs. Concordant AST resu...