experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. ejaculates were collected from four rams. in each session, second ejaculates (n=4) were collected in a tube containing 1 ml coating buffers which were prepared by 10, 15 or 20% egg yolk plus tris-fructose. samples were pooled, centrifuged and the supernatant removed in the labora...

triploidy in grass carp, ctenopharyngodon idella valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. two simplified methods explained for verification of triploidy in grass carp. the cold shock (7 ?c) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. in cold shock, the start point (2.0 min after fertilization) showed the ...

BACKGROUND Although cold shock responses and the roles of cold shock proteins in microorganisms containing multiple cold shock protein genes have been well characterized, related studies on bacteria possessing a single cold shock protein gene have not been reported. Thermoanaerobacter tengcongensis MB4, a thermophile harboring only one known cold shock protein gene (TtescpC), can survive from 5...

Triploidy in grass carp, Ctenopharyngodon idella Valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. Two simplified methods explained for verification of triploidy in grass carp. The cold shock (7 ?C) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. In cold shock, the start point (2.0 min after fertilization) showed th...

Sensing a sudden change of the growth temperature, all living organisms produce heat shock proteins or cold shock proteins to adapt to a given temperature. In a heat shock response, the heat shock sigma factor plays a major role in the induction of heat shock proteins including molecular chaperones and proteases, which are well-conserved from bacteria to human. In contrast, no such a sigma fact...

F OUR trials were conducted with boar spermatozoa to determine the effects of dilution rate, seminal plasma content and extender composition on susceptibility to cold shock and the development of resistance to cold during 30 C incubation. Acrosome morphology and sperm motility were used to assess damage by cold shock. The susceptibility of boar spermatozoa to cold shock increased as the dilutio...

During cellular adaptation to low temperature, Escherichia coli transiently synthesizes the major cold-shock protein CspA. It was found that adaptation to cold shock is blocked when the 143-base sequence of the 5' untranslated region (5' UTR) of the cspA mRNA is overproduced. The overproduction of this UTR at 15 degrees C caused the synthesis of not only CspA but also other cold-shock proteins ...

Rocky Mountain spotted fever is caused by Rickettsia rickettsii infection. R. rickettsii can be transmitted to mammals, including humans, through the bite of an infected hard-bodied tick of the family Ixodidae. Since the R. rickettsii genome contains only one cold-shock-like protein and given the essential nature of cold-shock proteins in other bacteria, the structure of the cold-shock-like pro...

Metabolic profiling analyses were performed to determine metabolite temporal dynamics associated with the induction of acquired thermotolerance in response to heat shock and acquired freezing tolerance in response to cold shock. Low-M(r) polar metabolite analyses were performed using gas chromatography-mass spectrometry. Eighty-one identified metabolites and 416 unidentified mass spectral tags,...