Two oxacillin disk methods were compared with a cefoxitin disk diffusion test for detection of methicillin-resistant Staphylococcus aureus (MRSA), with PCR for mecA as the reference method. When tested with 115 MRSA and 350 methicillin-susceptible S. aureus isolates, the cefoxitin disk test (specificity 100%, sensitivity 96.5%) was superior to the oxacillin disk methods (specificity 99.1%, sens...

In this study, 153 clinical isolates of Shigella were screened for extended-spectrum beta-lactamase (ESBL) production by double-disk synergy test. After being confirmed by the combined disk-test and inhibitor-combined E-test strips, all positive isolates were tested for the bla gene by PCR and the enzymes by isoelectric focusing. DNA sequencing of PCR products revealed that five Shigella sonnei...

results the mean of the inhibition zone diameter around the discs of green tea extract in all concentrations was significantly less than 1% sodium hypochlorite (p < 0.001). the antimicrobial effect increased in higher concentrations of extracts of green tea (p < 0.001). conclusions although the antimicrobial effect increases in higher concentrations of extracts of green tea, 1% sodium hypochlor...

Background & objective: Beta-lactam antibiotics resistance specifically Imipenem and Meropenem, the last choices of treatment, causes fatal events in patients with P.aeruginosa infection. The aim of this study was to detect the VIM and IMP of metallo-beta-lactamase genes in 103 isolates of P. aeruginosa in two Iranian hospitals. <strong...

Disk diffusion test is the usual applicable method for assessing the antimicrobial susceptibility pattern in most institutions and hospitals. The aim of this study was to determine the reliability of resistant-reported results of disk diffusion test for 6 routinely used antibiotics against Gram-positive microorganisms of nosocomial origin, using E-test method. Over a 1-year period, clinical spe...

The spread of multi-drug resistant tuberculosis necessitates the discovery of new classes of antibacterials and compounds that inhibit macromolecules involved in these resistant mechanisms. Thirty ethanol extracts from nineteen selected plants from Zimbabwe were screened against Mycobacterium aurum and Corynebacterium glutamicum using the agar disk diffusion method. These two organisms were use...