نتایج جستجو برای: conditioned medium

تعداد نتایج: 259755  

Journal: :The European respiratory journal 1997
M M Abdelaziz J L Devalia O A Khair C Rusznak M Calderon R J Sapsford H Bayram R J Davies

Although some studies have shown that long-term treatment of asthmatics with nedocromil sodium can reduce airway hyperresponsiveness and improve symptoms and lung function, the mechanisms underlying its effects are not well understood. We have investigated the effect of nedocromil sodium on eosinophil chemotaxis, eosinophil adherence to human endothelial cells and release of soluble intercellul...

Journal: :The Journal of Cell Biology 1990
R M Lyons L E Gentry A F Purchio H L Moses

Medium conditioned by Chinese hamster ovary (CHO) cells transfected with the simian pre-pro-TGF beta 1 cDNA contains high levels of latent TGF beta 1. The amino-terminal region of the TGF beta 1 precursor is secreted and can be detected in the conditioned medium by immunoblotting using peptide antibodies specific for amino-terminal peptides. Chemical cross-linking of CHO-conditioned medium usin...

Journal: :Investigative ophthalmology & visual science 1987
H C Wong M Boulton J Marshall P Clark

Long-term cultures of bovine retinal capillary endothelial cells (BRCEC) have been established using bovine retinal pericyte conditioned medium (PCM). PCM had greater mitogenic activity on BRCEC than unconditioned medium (P less than 0.01). The mitogenic activity of PCM was not cell specific since bovine Tenon's fibroblast conditioned medium also had significant activity. PCM's activity was not...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهرکرد - دانشکده دامپزشکی 1389

چکیده در این مطالعه به بررسی اثرات مفید حضور کشت اویداکت و یا ترشحات آن در مرحله ivf (in vitro fertilization) با استفاده از محیط sof (synthetic oviductal fluid) بر شاخص های رشد جنین گوسفند (میزان کلیواژ و میزان تولید و اتساع و هچ بلاستوسیست) پرداخته شده است. بدین منظور تخمک ها به صورت تصادفی در 5 گروه ivf شدند که عبارتند از: الف) ivf در محیط sof (گروه کنترل) ب) ivf در محیط sof در حضور کشت اوید...

2002
Ryuichi Tatsumi Akihito Hattori Yoshihide Ikeuchi Judy E. Anderson Ronald E. Allen

Application of mechanical stretch to cultured adult rat muscle satellite cells results in release of hepatocyte growth factor (HGF) and accelerated entry into the cell cycle. Stretch-activation of cultured rat muscle satellite cells was observed only when medium pH was between 7.1 and 7.5, even though activation of satellite cells was accelerated by exogenous HGF over a pH range from 6.9 to 7.8...

Journal: :Journal of cell science 1978
E F Rossomando B Maldonado E V Crean E J Kollar

At the onset of development, the single cells of the eukaryotic micro-organism Dictyostelium discoideum secrete proteolytic activity which can be assayed using the insoluble substrate remazolbrilliant blue hide. The activity is not secreted by exponentially growing cells, but does appear extracellularly at the onset of the stationary growth phase. When growth phase cells are resuspended in non-...

Journal: :Spine 2004
Lawrence M Boyd Jun Chen Virginia B Kraus Lori A Setton

STUDY DESIGN Matrix protein gene expression was determined for cells of the anulus fibrosus (AF) and nucleus pulposus (NP) regions of the intervertebral disc when cultured in AF or NP cell-conditioned medium. OBJECTIVES To investigate changes in mRNA levels for type I collagen, type II collagen and aggrecan in cells of the AF and NP in response to cell-conditioned medium. SUMMARY OF BACKGRO...

Journal: :Journal of animal science 1991
T L Roth K L White D W Horohov

Immunosuppressive activity of conditioned medium from cultured ovine, caprine, and hybrid trophoblast tissue was examined. Conceptuses were obtained from naturally mated donor ewes and does at d 20 of gestation and trophoblast tissue was cultured for 24 h in medium supplemented with 15% calf serum and 1% antibiotic/antimycotic. Conditioned medium was added to pokeweed mitogen-stimulated sheep a...

2017
Peng-Yu Chang Bo-Yin Zhang Shuang Cui Chao Qu Li-Hong Shao Tian-Kai Xu Ya-Qin Qu Li-Hua Dong Jin Wang

Microvascular injury initiates the pathogenesis of radiation enteropathy. As previously demonstrated, the secretome from mesenchymal stem cells contains various angiogenic cytokines that exhibited therapeutic potential for ischemic lesions. As such, the present study aimed to investigate whether cytokines derived from mesenchymal stem cells can repair endothelial injuries from irradiated intest...

Journal: :Indian journal of experimental biology 2006
Ravindra M Satpute Rajpal S Kashyap Rani Poonam Kainthla Hemant J Purohit Girdhar M Taori Hatim F Daginawala

Neurite outgrowth is essential for the communication of the nervous system. The rat Pheochromocytoma (PC12) cells are commonly used in the neuronal cell study. It is well known that exogenous stimuli such as Nerve Growth Factor (NGF) induce neurite outgrowth. In the present study it has been investigated whether or not the conditioned medium from human neuroblastoma cell line (IMR-32) and human...

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