Survival of the sclerotial parasite Coniothyrium minitans in soil, when applied as spore suspension or colonised solid substrate (maizemeal-perlite) inocula, and ability to infect Sclerotinia sclerotiorum sclerotia incorporated into the soil after different times was assessed over 6 months. Unambiguous detection of the C. minitans isolate from the indigenous C. minitans soil population was achi...

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A non-mycoparasitic restriction enzyme-mediated DNA integration (REMI) mutant of Coniothyrium minitans (R2427) contains two tandem plasmid copies integrated towards the 3' end of an ORF. The predicted polypeptide (845 aa) exhibits high similarity with DNA-helicase proteins from other filamentous fungi and yeasts that play a role in mitochondrial DNA maintenance and repair. Disruption of the C. ...

A Coniothyrium minitans strain (T3) co-transformed with the genes for beta-glucuronidase (uidA) and hygromycin phosphotransferase (hph), the latter providing resistance to the antibiotic hygromycin B, was used to investigate the survival and infection of sclerotia of Sclerotinia sclerotiorum by C. minitans over time in four different soils. Infection of sclerotia was rapid in all cases, with th...

Coniothyrium minitans (synonym, Paraphaeosphaeria minitans) is a highly specific mycoparasite of the wide host range crop pathogen Sclerotinia sclerotiorum. The capability C. to destroy sclerotia S. sclerotiorum has been well recognized and it available as widely used biocontrol product Contans WG. We present draft genome sequence Conio (IMI 134523), which previously in extensive studies that f...

Sclerotinia sclerotiorum is an important plant pathogenic fungus of many crops. Our previous study identified the S. agglutinin (SSA) that can be partially degraded by serine protease CmSp1 from mycoparasite Coniothyrium minitans. However, biological functions SSA in pathogenicity and its response to infection C. minitans, as well environmental stresses, remain unknown. In this study, disruptio...

The NADPH oxidase complex of a sclerotial mycoparasite Coniothyrium minitans, an important biocontrol agent against crop diseases caused by Sclerotinia sclerotiorum, was identified and its functions involved in conidiation and mycoparasitism were studied. Gene knock-out and complementary experiments indicated that CmNox1, but not CmNox2, is necessary for conidiation and parasitism, and its expr...