AIM To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7-dimethoxycoumarin (DMOC) on crosslink. METHODS Primary cultured rabbit kidney proximal tubular cells (PTC) were established. DNA interstrand crosslink was assayed with ethidium bromide binding and DNA-protein crosslink with 125I-postlabelling. PTC were incubated with cisplatin for 24 h. DMOC was p...

Experimental studies on epoxies report that the microstructure consists of highlycrosslinked localized regions connected with a dispersed phase of low-crosslink density epoxy. Because epoxies play a major role in many structural applications, the influence of the crosslink distribution on the thermo-mechanical properties must be determined. But as experiments cannot reliably report the exact nu...

Silane coupling agent generally use as improve the properties of white filler use in rubber compound for better dispersion. Also silane coupling agent reacts with organic filler & polymer chain as crosslink. Here in this research study the crosslink density & swelling phenomena of silane participate in vulcanization process with Nitrile rubber compound. Also show the comparisons of carbon fille...

The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely related 5-bromo-2'-deoxycytidine (BrdC). Our results showed that two types of cros...

M odel reactions o f the hardening o f gelatin with 13C labeled form aldehyde were m onitored by 13C N M R spectroscopy in solution and in the solid state. In solution prim ary attack o f form aldehyde first forms methylols o f lysine residues and later o f arginine residues, which react to a lysine-arginine crosslink. D uring the drying process o f hardened gelatin, in addition to the lysine-a...

Model silicone foul-release coatings with controlled molecular architecture were evaluated to determine the effect of compositional variables such as filler loading and crosslink density on pseudobarnacle attachment strength. Pseudobarnacle adhesion values correlated with filler loadings in both condensation and hydrosilylation-cured silicones. Variation of crosslink density of hydrosilylation-...

Objectives. To evaluate crosslink densities of two bulk fill composite resins and determine if the used Food Simulating Organic Solvent (FSOS) affected them. Methods. Forty specimens were prepared from SureFill and SonicFill bulk fill composite resins, 20 each. All specimens were stored dry for 24 h. Each group was divided into 2 subgroups: stored in ethanol (E) 75% or in methyl ethyl ketone (M...

Many carcinogenic as well as chemotherapeutic agents cause covalent linkages between complementary strands of DNA. If unrepaired, DNA crosslinks are blocks to DNA replication and transcription and therefore represent potentially lethal lesions to the cell. Genetic studies of Escherichia coli have demonstrated that the repair enzyme ABC excision nuclease, coded for by the three unlinked genes, u...

The crosslink density plays a key role in the mechanical response of the amorphous polymers in previous experiments. However, the mechanism of the influence is still not clear. In this paper, the influence of crosslink density on the failure behavior under tension and shear in amorphous polymers is systematically studied using molecular dynamics simulations. The present results indicate that th...

The interstrand photocrosslinking of a 5-chloro-2′-deoxy-4-thiouridine modified DNA oligonucleotide proceeds via two-step photocascade involving the formation thermally reversible crosslink and its conversion to stable fluorescent crosslink.