objective: different cryoprotectants are used for cryopreservation of ovarian tissue in patients at risk of infertility. ethylene glycol (eg), dimethyl sulfoxide (dmso) and propanediol (proh) have been chosen as the basic permeable cryoprotectants due to their decreased glass-formation characteristics compared to other cryoprotectants. in the present study, the effects of two different vitrific...

Populations of three isolates of Bursaphelenchus xylophilus, the pinewood nematode, and one of B. mucronatus were treated with three cryoprotectants at -70 C for 24 hours followed by deep freezing at -180 C in liquid nitrogen for different periods of time. A solution of 15% glycerol, 35% buffer S, and 50% M9, or 1% aqueous solution of dimethylsulfoxide (DMSO), or a mixture of 60% M9 and 40% S b...

The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...

The objective of this study was to investigate the effect of selected biopolymers on the rheological properties of surimi. In our paper, we highlight the functional properties and rheological aspects of some starch mixtures used in surimi. However, the influence of some other ingredients, such as cryoprotectants, mannans, and hydroxylpropylmethylcellulose (HPMC), on the rheological properties o...

Spermatozoa cryopreservation is an effective method for maintaining male fertility in humans. Nevertheless, there are some limitations of sperm cryopreservation, which called as cell injury by cryoprotectant, that cannot be avoided. This process will affect embryo quality. Therefore, it mandatory to modify cryoprotectant spermatozoa improve review aimed summarize the modification can damage cel...

Disorders related to human health involving proteins that form the gluten network are increasingly in society, justifying need offer bakery products for this market niche. This study aimed gather important information about challenges technological production gluten-free market. The approach encompasses development of frozen dough baking, use cryoprotectants maintain cell viability during freez...

ABSTRACT Aroeira-do-sertão is a Brazilian native species that has been widely explored. Thus, the population of this reduced and techniques for its preservation are essential, such as conservation seeds in liquid nitrogen (LN). The objective work was to evaluate different cryoprotectant solutions cryopreservation aroeira-do-sertão LN (-196 °C). treatments used were: control (7.5±1.5 °C); withou...

In the pharmaceutical industry, cryoprotectants are added to buffer formulations protect active ingredient from freeze- and thaw damage. We investigated freezing thawing of aqueous sodium citrate with various cryoprotectants, specifically amino acids (cysteine, histidine, arginine, proline lysine), disaccharides (trehalose sucrose), polyhydric alcohols (glycerol mannitol) surfactants (polysorba...

Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preserva...