Detailed and expressive e-learning specifications like IMS LD [1] are necessary to create efficient e-learning applications. E-learning specifications that meet these demands tend to be long and detailed such that authoring e-learning content becomes a tedious and error-prone endeavour. This paper describes DBAT-LD (Dialogue-Based Authoring of Learning Designs) which is a document generating di...

The time-course of expression of dbat and dbtnbt genes involved in the later steps of paclitaxel biosynthesis and the intracellular taxane accumulation were investigated through a 64-day subculture interval of VI/M1 and VI/M2 Taxus baccata callus cultures. HPLC proved traces of baccatin III and an intracellular content of paclitaxel up to 90 microg/g DW. The steady-state of the respective gene ...

The natural concentration of the anticancer drug Taxol is about 0.02% in yew trees, whereas that of its analogue 7-β-xylosyl-10-deacetyltaxol is up to 0.5%. While this compound is not an intermediate in Taxol biosynthetic route, it can be converted into Taxol by de-glycosylation and acetylation. Here, we improve the catalytic efficiency of 10-deacetylbaccatin III-10-O-acetyltransferase (DBAT) o...

The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5...

Performance metrics and evaluation criteria are used to develop a novel taxonomy which classifies barrier mechanisms into categories ranging from simple Static-Binding Idle-Tasking (SBIT) methods to robust Dynamic-Binding Any-Tasking (DBAT) methods. Such capabilities include support for multiple barriers, reconfigurable and reusable synchronization resources, and provisions for dynamic process...