objectives lipid components of spermatozoa have an important role in its functional activity. in this study the concentration of cholesterol, phospholipid, triacylglycerol and total lipid in different subsets of human spermatozoa, isolated as three fractions by a discontinuous puresperm gradient, was determined. methods aliquots of the liquefied semen samples (n=107) were layered on top of the ...

Objective Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGC) and other contaminating cell types. Several strategies such as the antibody binding methods, the flask method, the cell strainer, and the positive selection of granulosa aggregates after density gradient centrifugation, were tested as LGCs purification methods. Each of these strategies has its own advantages...

background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...

sperm selection techniques have became an important tool to improve sub fertile ejaculates. the main aim of this study was to assess the dynamics of motile sperm subpopulations of ejaculates from subfertile stallions subjected to density gradient centrifugation (dgc) involving a double layer colloid [dgc2], before and after storage for 24 and 48 hours. ejaculates from eight stallions with ferti...

background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...