نتایج جستجو برای: dhplc

تعداد نتایج: 390  

Journal: :Clinical chemistry 2004
Stefania Stenirri Isabella Fermo Stefania Battistella Silvia Galbiati Nadia Soriani Rita Paroni Maria Pia Manitto Elisabetta Martina Rosario Brancato Rando Allikmets Maurizio Ferrari Laura Cremonesi

BACKGROUND Mutations in the retina-specific ABC transporter (ABCA4) gene have been associated with several forms of macular degenerations. Because the high complexity of the molecular genotype makes scanning of the ABCA4 gene cumbersome, we describe here the first use of denaturing HPLC (DHPLC) to screen for ABCA4 mutations. METHODS Temperature conditions were designed for all 50 exons based ...

Journal: :Journal of molecular endocrinology 2006
Michel Crépin Pascal Pigny Fabienne Escande Catherine Cardot Bauters Alain Calender Sylvain Lefevre Marie-Pierre Buisine Nicole Porchet Marie-Françoise Odou

The identification of mutations in the MEN1 gene causing MEN1 has represented a challenge since the cloning of the gene in 1997 because of the lack of mutation hot-spots in the gene and the lack of phenotype-genotype correlations. The use of denaturing high performance liquid chromatography (DHPLC), a high throughput, reliable and automated heteroduplex-based technique, is the ideal for mutatio...

Journal: :Clinical chemistry 2004
Alessia Bagattin Caterina Veronese Barbara Bauce Wim Wuyts Luca Settimo Andrea Nava Alessandra Rampazzo Gian Antonio Danieli

BACKGROUND Mutations in the RYR2 gene, which encodes the cardiac ryanodine receptor, have been reported in patients showing either arrhythmogenic right ventricular cardiomyopathy, type 2, or stress-induced polymorphic ventricular tachycardia. Both clinical phenotypes are characterized by a high risk of sudden death. Detection of RYR2 mutations is particularly important because beta-blocker trea...

Journal: :Turkish journal of medical sciences 2014
Hülya Sümer Çelebı Hilal Özdağ

BACKGROUND/AIM Sensitive and cost-effective detection of point mutations is important in genetics research. Denaturing high-performance liquid chromatography (DHPLC) is known to be one of the most sensitive techniques for point mutation detection. A more recent technique, high-resolution melting (HRM), is based on the melting behavior of PCR products. In this study, the efficiency and sensitivi...

Journal: :Turkish journal of haematology : official journal of Turkish Society of Haematology 2011
Yücel Erbilgin Suzin Çatal Ahmet Emre Eşkazan Özden Hatırnaz Teoman Soysal Uğur Özbek

OBJECTIVE Despite the efficacy of the BCR-ABL tyrosine kinase inhibitor imatinib, the development of resistance against imatinib has been observed. The most important mechanisms known to cause resistance are point mutations in the ABL tyrosine kinase and the ATP domain. This study describes the use of denaturing high performance liquid chromatography (dHPLC) as a method to screen for mutations ...

2006
Patrick S. Callery Bernard Boswell Peter M. Gannett Robert L. Haining Madhu Sanga Padma Tirumalai Timothy S. Tracy

To provide better customer service, NCJRS has made this Federally-funded grant final report available electronically in addition to traditional paper copies. Opinions or points of view expressed are those of the author(s) and do not necessarily reflect the official position or policies of the U.S. Introduction Forensic DNA testing is seriously hampered by a growing backlog of DNA samples. There...

Journal: :Journal of clinical pathology 2005
B Yu N A Sawyer M Caramins Z G Yuan R B Saunderson R Pamphlett D R Richmond R W Jeremy R J Trent

AIMS To evaluate the usefulness of denaturing high performance liquid chromatography (DHPLC) as a high throughput tool in: (1) DNA mutation detection in familial hypertrophic cardiomyopathy (FHC), and (2) single nucleotide polymorphism (SNP) discovery and validation in sporadic motor neurone disease (MND). METHODS The coding sequence and intron-exon boundaries of the cardiac beta myosin heavy...

Journal: :Anticancer research 2005
Kalliopi Pazaitou-Panayiotou Athina Kaprara Leda Sarika Thanasis Zovoilis Ioulia Belogianni Iraklis Vainas Georgios Nasioulas

BACKGROUND Multiple Endocrine Neoplasia type 2 (MEN 2) is an autosomal dominant inherited syndrome characterized by a strong predisposition for developing endocrine tumors. MEN 2 is caused by germline mutations in the ret proto-oncogene. We investigated the feasibility of using the DHPLC technique in mutation detection of the ret gene in members of MTC families. We compared DHPLC analysis with ...

Journal: :Croatian medical journal 2003
Greggory S LaBerge Robert J Shelton Phillip B Danielson

AIM To determine the forensic utility for pairwise DNA comparisons and DNA mixture resolution with denaturing high-performance liquid chromatography (DHPLC) of human mitochondrial DNA (mtDNA). METHODS MtDNA hypervariable regions (HV) 1 and 2 from the mtDNA D-loop were amplified by the polymerase chain reaction and mixed between known and unknown sample sources. The DNA mixtures were denatured...

Hassan Dastsooz, Majid Fardaei, Nazanin Vahedi

Objective(s):  Denaturing high performance liquid chromatography (DHPLC) is a high throughput approach for screening DNA sequence variations. To assess oven calibration, cartridge performance, buffer composition and stability, the WAVE Low and High Range Mutation Standards are employed to ensure reproducibility and accuracy of the chromatographic analysis. The purpose of this study was to provi...

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