background: echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. the strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. using dna based methods for strain /genotype characterizations of e. granulosus have some difficulties, especi...

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with 154~155 bp. The sequence of ITS2 was mo...

A cDNA (pBIFl1) clone corresponding to the complete “10-kD precursor polypeptide associated with the PSII was isolated from the inflorescence cDNA library of Chinese cabbage (Brassica campestris L. spp. pekinesis). The nucleotide sequence was determined using the dideoxynucleotide chain-termination method (Sanger et al., 1977) and the amino acid sequence was deduced (Table I). The cDNA i s comp...

Bacteroides forsythus is known as a periodontopathogen associated with periodontitis, and it produces a tripsin-like protease, cell-death inducing factor, and sialidase (neuraminidase), as putative virulence factors. The purpose of this study was to clone the sialidase gene from B. forsythus ATCC 43037, and to analyze the biological characteristics. A positive clone (pHI-1) was successfully iso...

MOTIVATION Scientific data reside in a number of different data sources, both public and commercial, and are accessible through various software applications. Data and software interoperability is becoming a critical issue in the research community. In our opinion, the need to develop a unified object model for biotechnology must be recognized. Herein, we present a prototype and core elements f...

A novel type of 5-oxoprolinase was found in a cell extract of strain N-38A, which was later identified as Alcaligenes faecalis. The enzyme in the cell extract was purified to a homogeneous state with a yield of 16.6%. The molecular weight of the purified enzyme was estimated to be 47,000 by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration, suggesting that the en...

The 5' end or alpha-S1 casein promoter has a significant role in milk protein gene expression. The understanding of the translation process of alpha-S1 casein mutants will provide us an opportunity to make the best selection in livestock providing more proteins in milk. Blood samples were taken from three hundred of Naeinian goats and sheep, and DNA extraction was done using modified salting ou...

The aim of this study was to perform molecular and bioinformatics analysis of IGFBP2 gene promoter in association with some economic traits in indigenous Makuee (MS) and Lori-Bakhtiari (LB) breeds. DNA was extracted from blood samples of 120 MS and 200 LB and a 297 bp fragment from the upstream sequences of studied gene was amplified and genotyped by single-strand conformational polymo...