A screening procedure is presented which allows the isolation of yeast mutants (typ tir) with highly efficient utilization of exogenous deoxythymidine-5'-monophosphate (5'-dTMP) (greater than 50%). Data are given concerning the phenomenon of 5'-dTMP utilization in general: (i) The ability of S. cerevisiae to incorporate exogenous 5'-dTMP was found to already to be a wild type feature of this ye...

During the infection by bacteriophage T4, dTMP and 5hydroxymethyl dCMP (HmdCMP) are synthesized in the ratio of 2:1, respectively, i.e. at the Thy/HmCyt ratio found in T4 DNA. This study demonstrates that on infection by a cd (phage-induced dCMP deaminase) mutant the dTMP/ HmdCMP synthesis ratio is approximately 0.6. The ratio remains about 0.6 on infection by cd dna double mutants. At the same...

It was found that there are two kinds of pyrimidine nucleoside, monophosphokinase deoxythymidine 5'-monophosphate-deoxyuridine 5'-monophosphate (dTMP-dUMP) kinase and cytidine 5'-monophosphate-deoxycytidine 5'-monophosphate-uridine 5'-monophosphate-doexyuridine 5'-monophosphate (CMP-dCMP-UMP-dUMP) kinase, and their molecular weights were calculated to be 46,000 and 26,000, respectively, by gel ...

Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium. The sot1 mutation maps between rad1 and the centromere of chromosome XVI, an...

Autosomal recessive mutations in the thymidine kinase 2 gene (TK2) cause mitochondrial DNA depletion, multiple deletions, or both due to loss of TK2 enzyme activity and ensuing unbalanced deoxynucleotide triphosphate (dNTP) pools. To bypass Tk2 deficiency, we administered deoxycytidine and deoxythymidine monophosphates (dCMP+dTMP) to the Tk2 H126N (Tk2(-/-)) knock-in mouse model from postnatal ...

In this paper, we consider the compressed video background subtraction problem that separates and foreground of a from its measurements. The usually lies in low dimensional space is sparse. More importantly, each frame natural image has textural patterns. By exploiting these properties, develop message passing algorithm termed offline denoising-based turbo (DTMP). We show structural properties ...

The present study explores the use of alpha-particle-emitting, bone-seeking agents as candidates for targeted radiotherapy. Actinium and thorium 1,4,7,10 tetraazacyclododecane N,N',N'',N''' 1,4,7,10-tetra(methylene) phosphonic acid (DOTMP) and thorium-diethylene triamine N,N',N'' penta(methylene) phosphonic acid (DTMP) were prepared and their biodistribution evaluated in conventional Balb/C mic...

The hyperthermophilic anaerobic archaeon Pyrococcus abyssi, which lacks thymidine kinase, incorporates label from extracellular uracil, but not from thymidine, into its DNA. This implies that P. abyssi must synthesize dTMP (thymidylate), an essential precursor for DNA synthesis, de novo. However, iterative similarity searches of the three completed Pyrococcus genomes fail to detect candidate ge...

The importance of this pathway to E. coli for the synthesis de nouo of dTMP becomes apparent from studies indicating only a dubious contribution toward the formation of dTMP from Reaction Sequence 1, because of the low levels of deoxycytidylate deaminase in this organism (5). It remains to be seen whether an analogous pathway to Reaction Sequence 2 can be observed in animal tissues. Some suppor...