نتایج جستجو برای: e1a

تعداد نتایج: 1663  

ژورنال: :پژوهش های آسیب شناسی زیستی 0
haleh vosgha department of genetics, faculty of basic sciences, tarbiat modares university, tehran, iran mehrdad behmanesh department of genetics, faculty of basic sciences, tarbiat modares university, tehran, iran majid sadeghizadeh department of genetics, faculty of basic sciences, tarbiat modares university, tehran, iran

هدف: انکوپروتئین e1a در آدنوویروس تیپ 5 یک فاکتور تنظیمی است که موجب کنترل فرایند رونویسی ژن های آدنوویروس می شود. این پروتئین با تغییر در عملکرد پروتئین های مهم سلولی از جمله p21 و prb سبب ایجاد شرایط مساعد برای همانندسازی ژنوم ویروس و ترانسفورم شدن سلول های میزبان می شود. هدف از تحقیق حاضر مهار پایدار بیان ژن e1a در سلول های hek 293 با استفاده از روش rnai بوده تا آثار این مهار روی سلول های فو...

Journal: :modares journal of medical sciences: pathobiology 2008
haleh vosgha mehrdad behmanesh majid sadeghizadeh

objective: e1a oncoprotein of adenovirus type 5 is a regulatory factor which controls transcription of other adenovirus genes. this protein promotes both viral genom replication and host cell transformation by altering the function of certain important cellular proteins such as p21 and rb. the aim of the present study was to constantly reduce the expression of e1a gene in hek 293 cell line by r...

Journal: :Cancer research 2003
Tiejun Zhao Xiao-Mei Rao Xiaoming Xie Ling Li Timothy C Thompson Kelly M McMasters Heshan Sam Zhou

The adenovirus E1A proteins are involved in the transcriptional activation of viral and cellular genes needed for controlling cell cycle and virus replication. Undifferentiated embryonic carcinoma cells have the ability to produce an E1A-like activity that can induce the expression of E1A-targeted adenoviral and cellular genes in the absence of the E1A products. Differentiated embryonic carcino...

Journal: :Journal of virology 2010
Jessica Komorek Mohan Kuppuswamy T Subramanian S Vijayalingam Elena Lomonosova Ling-Jun Zhao Joe S Mymryk Kimberly Schmitt G Chinnadurai

The adenovirus (Adv) oncoprotein E1A stimulates cell proliferation and inhibits differentiation. These activities are primarily linked to the N-terminal region (exon 1) of E1A, which interacts with multiple cellular protein complexes. The C terminus (exon 2) of E1A antagonizes these processes, mediated in part through interaction with C-terminal binding proteins 1 and 2 (CtBP1/2). To identify a...

Journal: :American journal of physiology. Lung cellular and molecular physiology 2004
Emiko Ogawa W Mark Elliott Fiona Hughes Thomas J Eichholtz James C Hogg Shizu Hayashi

Previous studies showed an association between latent adenoviral infection with expression of the adenoviral E1A gene and chronic obstructive pulmonary disease (COPD). The present study focuses on how the adenoviral E1A gene could alter expression of growth factors by human bronchial epithelial (HBE) cells. The data show that connective tissue growth factor (CTGF) and transforming growth factor...

Journal: :Cancer research 2004
Yong Liao Mien-Chie Hung

The adenoviral type 5 E1A protein has been shown to induce sensitization to different categories of anticancer drug-induced apoptosis, partly by down-regulation of the activity of a critical oncogenic kinase Akt in both normal fibroblasts and epithelial breast cancer cells. Currently, the adenoviral E1A gene is being tested as an antitumor gene in multiple clinical trials. However, molecular me...

Journal: :Journal of virology 1988
D Bellgrau T A Walker J L Cook

The experiments described in this report were designed to examine whether target cells transfected with the adenovirus E1A gene and exhibiting increased susceptibility to lysis by natural killer cells and activated macrophages (J. L. Cook, T. A. Walker, A. M. Lewis, Jr., H. E. Ruley, F. L. Graham, and S. H. Pilder, Proc. Natl. Acad. Sci. USA 83:6965-6969, 1986) also express E1A proteins on thei...

Journal: :The EMBO journal 1998
K Alevizopoulos B Catarin J Vlach B Amati

We show here that the adenovirus E1A oncoprotein prevents growth arrest by the CDK2 inhibitor p27(Kip1) (p27) in rodent fibroblasts. However, E1A neither binds p27 nor prevents inhibition of CDK2 complexes in vivo. In contrast, the amount of free p27 available to inhibit cyclin E/CDK2 is increased in E1A-expressing cells, owing to reduced expression of cyclins D1 and D3. Moreover, E1A allows ce...

2016
Lin Fang Qian Cheng Jingjing Zhao Yan Ge Qi Zhu Min Zhao Jie Zhang Qi Zhang Liantao Li Junjie Liu Junnian Zheng

The conserved regions (CR) of adenoviral E1A had been shown to be necessary for disruption of pRb-E2F transcription factor complexes and induction of the S phase. Here we constructed a mutant adenoviral E1A with Rb-binding ability absent (E1A 30-60aa and 120-127aa deletion, mE1A) and investigated its antitumor capacities in vitro and in vivo. The mE1A suppressed the viability of tumor cells as ...

Journal: :The Journal of Experimental Medicine 2005
John M. Routes Sharon Ryan Kristin Morris Rayna Takaki Adelheid Cerwenka Lewis L. Lanier

The expression of the Adenovirus serotype 5 (Ad5) E1A oncogene sensitizes tumor cells to natural killer (NK) cell-mediated killing and tumor rejection in vivo. These effects are dependent on the ability of E1A to bind the transcriptional coadaptor protein p300. To test the hypothesis that E1A up-regulates ligands recognized by the NKG2D-activating receptor, we stably transfected the highly tumo...

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