Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total commun...

The chicken egg possesses physical and chemical barriers to protect the embryo from pathogens. OCX-36 (ovocalyxin-36) was suggested to be a 36 kDa eggshell-specific protein that is secreted by the regions of the oviduct responsible for eggshell formation. Its expression is strongly up-regulated during shell calcification. This protein was also detected in vitelline membrane and expressed in gut...

background: campylobacter spp. are the common pathogens that infect human beings via food. these bacteria are vibrio and have been implicated in abortion. serotyping is the best way for typing with penner scheme. c. jejuni and c. coli have 65 serotypes. c. coli is common in birds and dogs. due to high rate of prevalence of campylobacter in red-meat, chicken-meat and egg-shell, a suitable method...

This paper presents algorithms based on image processing for detecting internal blood spots and eggshell dirt by processing acquired images from eggs under different illuminations. The algorithm can also detect the severity of dirt on eggshell. In order to carry out image processing and extract useful features of captured images of eggs by machine vision we developed an algorithm in HSI color s...

Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a membe...

Bacterial penetration shortly after inoculation has not been reported extensively. Four strains of bacteria, Salmonella typhimurium (ST), Escherichia coli (EC), Pseudomonas earuginosa (PE) and Stapylococcus aureus (SA), in chicken egg components of Bovan brown were analyzed by serial dilution and scanning electron microscopy. After exposing the fresh eggs to the bacterial strains at concentrati...

Two experiments were conducted that confirmed the hypothesis that a dietary bicarbonate supplement will improve eggshell quality in hens at high temperatures as long as feed is consumed during the period of eggshell formation. End-of-lay hens were maintained on continuous light at temperatures of 30 and 35 C. Individual egg weights and shell quality measures for each hen were calculated as a pr...

Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of develo...