introduction: pronuclear size, morphology and orientation are known to affect embryo quality and implantation. the present study was aimed at evaluation of the possible relationship between protamine deficiency with pronuclear morphology and also cleavage and embryo quality. material and methods: semen sample of 160 candidate icsi and ivf couples were analyzed for sperm concentration, and motil...

the aim of this study was to examine the possible morphological and morphometric changes resulting from vitrification of embryos at the cleavage stage. in this study, 30 mice early-cleavage embryos at different stages of cleavage, resulting from in vitro fertilization (ivf) techniques, were examined before and after vitrification. digital images were taken from embryos before and after vitrific...

abstract background: research studies on reproductive mechanism of laboratory animals are essential for further advancement of assisted reproductive techniques (art). one of these studies includes the assessment of in-vitro development of pre-implantation embryos. the objective was to compare the cleavage rates and morphology of in-vivo formed 2 to 8 cell embryos and blastocysts with in-vitro c...

UNLABELLED BACKGROUND The assessment of the embryo quality is crucial to maintain an high pregnancy rate and to reduce the risk of multiple pregnancy. The evaluation of the pronuclear and nucleolar characteristics of human zygote have been proposed as an indicator of embryo development and chromosomal complement. The aim of the current study was to assess the role of pronuclear morphology ev...

BACKGROUND In human reproduction, embryo implantation is complex and poorly understood. At present, no single markers are used in routine treatment to assay biochemical functions of the human embryo. Soluble human leukocyte antigen-G (sHLA-G) could be considered a possible marker of embryo developmental potential. It is localized primarily on the extravillous trophoblast, making this antigen a ...

introduction: to evaluate and compare efficiency of sil-select with percoll to recover sperm with normal chromatin in morphology and also studyeffect of these parameters on fertilization, embryo quality and cleavagescore. materials and methods: assessment of semen parameters, protamine deficiency (cma3) and excessive histone (aniline blue staining) were carried out before and after sperm proces...

STUDY QUESTION Is the selection of a single Day 3 embryo by metabolomic profiling of culture medium with near-infrared (NIR) spectroscopy as an adjunct to morphology able to improve live birth rates in IVF, compared with embryo selection by morphology alone? SUMMARY ANSWER The live birth rate after embryo selection by NIR spectroscopy and morphology is not significantly different compared wit...