The genetic relationship among the Escherichia coli pathotypes was investigated. We used random amplified polymorphic DNA (RAPD) data for constructing a dendrogram of 73 strains of diarrheagenic E. coli. A phylogenetic tree encompassing 15 serotypes from different pathotypes was constructed using multilocus sequence typing data. Phylogram clusters were used for validating RAPD data on the clona...

Fifty-five Escherichia coli strains belonging to enteropathogenic E. coli (EPEC) serogroups were examined for phenotypic and genetic factors associated with virulence. The strains were isolated in Italy from children with diarrhea and identified as EPEC by clinical laboratories using commercially available antisera. O:H serotyping showed that 35 strains (27 of O26, O111, and O128 serogroups) be...

background: the purpose of this study was to find out the frequency of different serotypes of enteropathogenic es­cherichia coli (epec) among healthy/diarrheal cases. methods: a total of 191 strains, 111 from diarrheal and 80 from asymptomatic persons were examined. determination of the epec sero­groups was performed by agglutination tests using polyvalent and monovalent o antiserum. pcr-rflp a...

The attaching effacing (AE) adherence property is now recognized as an important virulence characteristic of enteropathogenic Escherichia coli (EPEC). The fluorescent-actin staining (FAS) test (S. Knutton, T. Baldwin, P. H. Williams, and A. S. McNeish, Infect. Immun. 57:1290-1298, 1989), which is diagnostic for the AE lesions produced by EPEC (and Vero cytotoxin-producing E. coli), has provided...

Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) possess a filamentous type III secretion system (TTSS) employed to deliver effector proteins into host cells. EspA is a type III secreted protein which forms the filamentous extension to the TTSS and which interacts with host cells during early stages of attaching and effacing (A/E) lesion formation. By immunofluoresc...

Stool specimens of patients with diarrhea or other gastrointestinal alterations who were admitted to Xeral-Calde Hospital (Lugo, Spain) were analyzed for the prevalence of typical and atypical enteropathogenic Escherichia coli (EPEC). Atypical EPEC strains (eae+ bfp-) were detected in 105 (5.2%) of 2015 patients, whereas typical EPEC strains (eae+ bfp+) were identified in only five (0.2%) patie...

Enteropathogenic Escherichia coli (EPEC), a leading cause of infantile diarrhea worldwide, includes 12 O serogroups expressing mainly flagellar antigens H2, H6 and H34. Other serotypes are known, but their role in diarrhea has not been firmly established (4). Typically, EPEC strains of these serotypes harbor a pathogenicity island, designated LEE (for locus of enterocyte effacement), that conta...

Results: Diarrhoeagenic E. coli was found in 148 (13.2 %) of examined specimens. Among these 77 (52 %) were enteropathogenic (EPEC), 69 (46.6 %) were enterotoxigenic (ETEC) and 2 (1.4 %) were enteroinvasive Escherichia coli (EIEC). EPEC serotypes 0119K69, 0114K90 and 0111K58 were the more frequent among twelve other serotypes. Diarrhoeagenic Escherichia coli showed high rates of resistance to a...

Typical and atypical enteropathogenic Escherichia coli (EPEC) strains differ in several characteristics. Typical EPEC, a leading cause of infantile diarrhea in developing countries, is rare in industrialized countries, where atypical EPEC seems to be a more important cause of diarrhea. For typical EPEC, the only reservoir is humans; for atypical EPEC, both animals and humans can be reservoirs. ...

BACKGROUND/AIM The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients. MATERIALS AND METHODS A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to ...