Chromosomal studies of bone-marrow cells have been reported on 30 patients with erythroleukaemia (Table I). Some of these reports give only meagre cytogenetic details, but there was an increased degree of polyploidy in at least 9 of the patients, significant aneuploidy in 12, and structural abnormalities in 11. Some patients showed more than one of these features, and only 8 patients had normal...

In an effort to activate the globin genes of non-erythroid cells, tetraploid murine erythroleukaemia cells (Friend cells) were fused with diploid human amniotic fibroblasts. When the Friend cells were pretreated with dimethylsulphoxide, an average of 27% heterokaryons was observed. These cells stained with benzidine, an indication that they contained haemoglobin. The cells incorporated radioact...

the characteristics of cellular and molecular mechanisms associated with cell proliferation and differentiation is important to understand malignancy. in this report we characterise a leukemic model, d5a1, to study the action of differentiation agent, cellular events and gene expression of the selected transcription factors. cells induced with 4 mm hexamethylene bisacetamide (hmba) caused signs...

Exposure of cultured human hepatoma cells (Hep G2) to medium containing 2% (v/v) dimethyl sulphoxide resulted in an approximate doubling in the activity of 6-aminolaevulinate dehydratase, an increase in the haem content and a decreased growth rate; induced enzyme activity was decrease by 50% after treatment with a-amanitin. The findings are strikingly similar to those seen in murine Friend-viru...

Leukaemia has been reported' recently in association with apparently unrelated haematological malignancies in patients treated with cytotoxic drugs. These leukaemias are mainly acute myeloid or variants such as acute myelomonocytic3 or erythroleukaemia.' We describe a case of chronic granulocytic leukaemia (CGL) developing in a patient with a non-Hodgkin's lymphoma treated with alkylating agent...

A case of systemic lupus erythematosus is described in which for 10 years the only significant findings were erythema multiforme and vasculitis. Gross hepatosplenomegaly with persistent pancytopenia developed, and bone marrow examination revealed the presence of sideroblasts. The patient's condition deteriorated, and subsequently she developed a severe bleeding tendency, terminating in erythrol...