A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by ...

MOTIVATION To make effective use of the vast amounts of expressed sequence tag (EST) sequence data generated by the Merck-sponsored EST project and other similar efforts, sequences must be organized into gene classes, and scientists must be able to 'mine' the gene class data in the context of related genomic data. RESULTS This paper presents the Merck Gene Index browser, an easily extensible,...

paris polyphylla smith var. yunnanensis (liliaceae) is an important traditional medicinal plant of the yunnan province in china. however, the genomic information regarding this plant is limited. to further understand its molecular background, we conducted illumina hiseq 2000 second-generation sequencing of this plant species. approximately 30,198,679 reads with an average length of 202 bases we...

In a first step toward understanding the molecular basis of pineapple fruit development, a sequencing project was initiated to survey a range of expressed sequences from green unripe and yellow ripe fruit tissue. A highly abundant metallothionein transcript was identified during library construction, and was estimated to account for up to 50% of all EST library clones. Library clones with metal...

The Expressed Sequence Tag (EST) division of GenBank, dbEST, is a large repository of the data being generated by human genome sequencing centers. ESTs are short, single pass cDNA sequences generated from randomly selected library clones. The approximately 415 000 human ESTs represent a valuable, low priced, and easily accessible biological reagent. As many ESTs are derived from yet uncharacter...

Expressed sequence tags (ESTs) are short sequence fragments of genes and may be used in genomic and genetic investigations. Despite rapid expansion of EST generation process, the resulting sequences are relatively low quality fragments and need to be cleaned before assembling into a larger sequence by identifying overlaps between sample sequences. EST comparative analysis and functional assignm...

BACKGROUND We investigate the usefulness of expressed sequence tags, ESTs, for establishing divergences within the tree of placental mammals. This is done on the example of the established relationships among primates (human), lagomorphs (rabbit), rodents (rat and mouse), artiodactyls (cow), carnivorans (dog) and proboscideans (elephant). METHODOLOGY/PRINCIPAL FINDINGS We have produced 2000 E...

We investigated the utility of the Gossypium arboreum EST sequences in the GenBank database for developing PCR-based markers targeting known-function genes in cultivated tetraploid cottons, G. hirsutum and G. barbadense. Four hundred sixty-five randomly selected ESTs from this library were subjected to BLASTn search against all GenBank databases, of which putative function was assigned to 93 ES...

Amplified fragment length polymorphism (AFLP) is often used for genetic mapping and diversity analysis, but very little information is currently available on their sequence characteristics. Species-specific sequences were analyzed from a single Coffea genome (Coffea pseudozanguebariae) associated with clustered or nonclustered AFLP loci of known genetic position. Compared with the expressed seq...

The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme...