In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

cumin (cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in iran. the purpose of this study was to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. for this purpose the cumin seeds (kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on ms basal medium. leaf and hyp...

BACKGROUND Explant browning presents a major problem for in vitro culture, and can lead to the death of the explant and failure of regeneration. Considerable work has examined the physiological mechanisms underlying Phalaenopsis leaf explant browning, but the molecular mechanisms of browning remain elusive. In this study, we used whole genome RNA sequencing to examine Phalaenopsis leaf explant ...

The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats foll...

An efficient protocol was established for rapid in vitro propagation of Stevia rebaudiana Bertoni, using explants from mature plants. The effect of 1/2MS, MS and B5 culture media, also 27 different combinations of auxins and cytokinins was studied on in vitro growth and development of Stevia rebaudiana Bertoni. The best culture medium for shoot and root formation was 1/2MS, producing 1.30 shoot...

We report combination of explants and enzymatic protocol as mixed enzymatic-explant procedure to faster extraction of MSCs from WJ. Umbilical cords (UC) were collected from Imam Khomini Hospital. For explant outgrowth, 6 9 pieces of WJ were transferred onto tissue culture flask and waited for attachment. For mixed enzymatic-explant, 1 cm pieces WJ were placed in enzymatic cocktail comprising 4 ...

The influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘Bianca’ and ‘El Torro’. Third, fourth and fifth nodal explants were cultured on the modified MS medium supplemented with 1.0 and 5.0 mg/l BA. In both the cultivars, higher rate of proliferation (‘Bianca’ 3.75; ‘El Torro’ 2.65) were obtained from the explants distal to the a...

N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic ...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

Previous studies reported that hepatitis B virus (HBV) deoxyribonucleic acid (DNA) can be detected in livers of patients who received transplants for hepatitis B despite the absence of serological markers of HBV recurrence. Quantification of HBV DNA was not performed and presence of covalently closed circular (ccc) DNA was not analyzed in most studies. We aimed to quantify total and ccc HBV DNA...