Non-targeted strategies based on high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and fluorescence (HPLC-FLD) fingerprints were evaluated to accomplish the classification authentication of instant coffee (40 samples), decaf (26 chicory (22 samples, including both ground instant), as well detect quantify frauds adulteration by multivariate chemometric methods. HPLC-UV ...

In all, 3872 urine specimens were screened for lysergic acid diethylamide (LSD) using the CEDIA DAU LSD assay. Forty-eight samples, mainly from psychiatric patients or drug abusers, were found to be LSD positive, but only 13 (27%) of these could be confirmed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) following immunoaffinity extraction (IAE). Additional ana...

This study describes the determination of polycyclic aromatic hydrocarbons (PAHs) in water using high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FLD). Because individual PAHs are generally present in water only at trace levels, a sensitive and accurate determination technique is essential. The separation and detection of five PAHs were run completely within 25...

Risk assessment of polycyclic aromatic hydrocarbons (PAHs) contaminated soil and source apportionment require accurate analysis of the concentration of each PAH congener in the soil. However, determination of low level PAH congeners in soil is difficult because of similarity in the chemical properties of 16 PAHs and severe matrix interferences due to complex composition of soils. It is therefor...

Flavanols are a class of polyphenols that have become the focus much research because their medicinal properties. For this paper, new method to determine in nutraceutical samples was developed, based on solid-liquid extraction and further analysis by liquid chromatography. Analytes were recovered ultrasound-assisted extraction, using mixture methanol/water/hydrochloric acid (70:29:1, v:v:v) as ...

A single step analysis method for the quantification of 11 different aromatic compound in aqueous samples by use HPLC-FLD/DAD.

A sensitive, specific, reproducible and optimized high performance liquid chromatography with fluorescence detection (HPLC-FLD) method for the determination of bergapten in rat plasma was established and applied to the pharmacokinetic and bioavailability study in rat after oral and intravenous administration of bergapten. The method was also successfully applied to the excretion study of bergap...

The accumulation of antibiotics by plants is a currently concern associated to yield reduction and transference antibiotic residues along the food web. Maize staple in many parts world; it also considered an important ingredient animal nutrition. Considering lack validated analytical methods for analysis ciprofloxacin (CIPRO) enrofloxacin (ENRO) shoot Zea mays maize, we developed reliable rever...

During the high-performance liquid chromatographic (HPLC) analysis of o-phthaldialdehyde (OPA)-derivatized amino acids such as arginine and homoarginine, we observed that the response of the fluorescence detection (FLD) system is decreased when the photodiode array detection (DAD) system (located before the FLD system) was simultaneously switched on. The decrease in the FLD response, i.e., the ...

We present a new method for the rapid analysis of ochratoxin A (OTA) in pig tissues (muscle, liver and kidney) using enzymatic digestion (ED) coupled to high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). OTA was digested with a 1% pancreatin solution in a phosphate buffer and then cleaned with ethylacetate. After being evaporated to dryness and re-dissolved, the sam...