A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p< 0.0001. This result correlates significantly with the standard ELISA assay, p<0....

Many areas of spectroscopy have benefited from the use of laser radiation sources. I present a discussion of the basic properties of the laser and how these properties can be advantageous when laser excitation is used in fluorometry. Although the laser has not yet been accepted as a routine instrument in the clinical laboratory, its unique properties have rendered it useful in several analytica...

In a continuing effort to develop inexpensive source tracking methods to detect human fecal contamination in environmental waters, we combined targeted sampling with fluorometry. Targeted sampling works by identifying hotspots of fecal contamination through multiple samplings over everdecreasing distances. Fluorometry identifies human fecal contamination by detecting optical brighteners, primar...

background and objective: oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. total antioxidant capacity plays a significant role in the body’s antioxidant defense, so its assessment is a matter of the utmost importance. currently, assessment of this capacity is performed in various s...

Potential sperm limitation has become a concern for blue crabs Callinectes sapidus in Chesapeake Bay. However, methods to quantify sperm in blue crabs are time intensive, which limits the sample size that can be obtained for sperm limitation studies. Our goal was to develop an efficient method for rapidly preparing spermathecae samples that more quickly quantifies sperm in blue crabs. Ultimatel...

Many bacterial source tracking (BST) methods are too expensive for most communities to afford. In an effort to develop an inexpensive method of detecting human sources of fecal contamination in a freshwater creek during baseflow and stormflow conditions, we combined targeted sampling with fluorometry. Targeted sampling is a prelude to BST and finds sources of fecal contamination by continued sa...

Fluorometry is used to detect intrinsic flavoprotein (FP) and nicotinamide adenine dinucleotide (NADH) signals in an open-chest rabbit model of myocardial ischemia-reperfusion injury. Myocyte apoptosis has been shown clinically to contribute to infarct size following reperfusion of ischemic myocardium. A noninvasive means of assessing apoptosis in this setting would aid in the treatment of subs...

Fluorescence anomalous phase advance (FAPA) is a newly discovered spectroscopy phenomenon: instead of lagging behind the modulated light, fluorescence signal can exhibit FAPA as if it precedes the excitation source in time. While FAPA offers a promising technique for probing dark state lifetime, the underlying mechanism is not fully elucidated. Herein we investigate frequency-domain phase fluor...