Preservation of living tissue and organs are very important to ensure successful transplantation. The storage of living tissue, from the time of removal of the donor tissue until transplantation, is one of the most important factors for successful tissue transplantation. The purpose of living tissue storage is to maintain the viability of the mammalian cells, and various methods have been devel...

Information on thermal history of skin tissue and iceball formation is critical for successful cutaneous cryosurgery. This paper presents a numerical analysis of tissue freezing during skin cancer treatment with liquid nitrogen (LN2). A simplified mathematical model is constructed by including the bio-physical processes of the freezing tissues and quantifying LN2 spray cooling with a convective...

Activated oxygen or oxygen free radicals have been implicated in a number of physiological disorders in plants including freezing injury. Superoxide dismutase (SOD) catalyzes the dismutation of superoxide into O2 and H2O2 and thereby reduces the titer of activated oxygen molecules in the cell. To further examine the relationship between oxidative and freezing stresses, the expression of SOD was...

Measurements of the electrical mobility of washed rabbit red cells and of ghosts produced by hypotonic solutions, freezing-and-thawing, chloroform, and saponin were made in the Abramson horizontal microelectrophoresis cell. These different forms of lysis, which corresponds to a variety of degrees of injury to the red cell, are unaccompanied by any change in electrical mobility. These observatio...

The problem of freezing will be considered from two standpoints: first, the local lesion and the therapeutic management of the frozen member, and second, the general effects of freezing including the possible presence of secondary shock and depression of the general body temperature. The literature on freezing as compared with burns is not very extensive. This may be accounted for by the fact t...

During cold acclimation, winter rye (Secale cereale L. cv Musketeer) plants accumulate antifreeze proteins (AFPs) in the apoplast of leaves and crowns. The goal of this study was to determine whether these AFPs influence survival at subzero temperatures by modifying the freezing process or by acting as cryoprotectants. In order to inhibit the growth of ice, AFPs must be mobile so that they can ...

Bulb onion (Allium cepa) is the second most widely cultivated and consumed vegetable crop in the world. During winter, cold injury can limit the production of bulb onion. Genomic resources available for bulb onion are still very limited. To date, no studies on heritably durable cold and freezing tolerance have been carried out in bulb onion genotypes. We applied high-throughput sequencing techn...