One of the most efficient mechanisms by which plants protect themselves from invading virusesis the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing(PTGS). In this mechanism, resistance to a virus is engineered through the expression of asegment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the mostdamaging viruses of potato, infecting...

conclusions by employment of gene optimization strategies, use of viral-based vectors and suppression of plant-derived gene silencing effect, efficient transient expression of hcvcp in tobacco with proper antigenic properties could be possible. background hepatitis c virus (hcv) is major cause of liver cirrhosis in humans. hcv capsid (core) protein (hcvcp) is a highly demanded antigen for vario...

ABSTRACT Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS rubber tree not yet reported. In this study, we described efficient by VIGS. The encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified genome. Small interfering RNAs from HbPDS and fragment were predicted length 399 bp sele...

objective: e-cadherin is widely down-regulated and tightly associated with tumor invasion and metastasis in multiple human cancer types. recent studies have shown that aberrant methylation of the e-cadherin gene promoter contributes to its silencing. however, information regarding epigenetic inactivation of e-cadherin in colorectal cancer is insufficient. herein, we correlate association of the...

To explore the regulation and potential mechanism of long non-coding ribonucleic acid taurine upregulated gene 1 on apoptosis cell cycle osteoblasts is main purpose. The overexpression plasmid small interfering were used to regulate expression in osteoblasts. was detected by quantitative reverse transcription polymerase chain reaction effect flow cytometry, western blot analyses detect division...