Hospital-acquired infections (HAI) increase morbidity and mortality and constitute a high financial burden on health care systems. An effective weapon against HAI is early detection of potential outbreaks and sources of contamination. Such monitoring requires microbial typing with sufficient reproducibility and discriminatory power. Here, a microbial-typing method is presented, based on Raman s...

Information on the oceanic migration patterns and relative marine survival of individual stocks is critical to our understanding of fluctuations of salmonid populations under changing climatic conditions. Migrations following stock-specific corridors may lead to differing marine survival and varying rates of return among stocks during periods of changing marine conditions. By comparing genetic ...

The β-thalassemias are genetic disorder caused by more than 200 mutations in the β-globin gene, resulting in a total (β0) or partial (β+) deficit of the globin chain synthesis. The most frequent Mediterranean mutations for β-thalassemia are: β039, β+IVSI-110, β+IVSI-6 and β0IVSI-1. Several molecular techniques for the detection of point mutations have been developed based on the amplification o...

conclusions the most predominant vaca genotypes in our areas were s1/m2 and s1/m1, which regard as the genotypes with more virulence intensity. the h. pylori vacas1a, caga genotypes have a significant relationship with the presence of pud and gc in iranian patients with dyspepsia. results in this study, vaca genotypes s1/m2, s1/m1, s2/m2 and s2/m1 were determined in 43.4%, 19.3%, 13.2% and 6% o...

the aim of this study was to detect the genotype of fasciola spp. in meshkin-shahr, ardabil province, northwestern iran in different hosts using pcr-rflp.the parasite hosts included cattle, and sheep. overall, 70 adult flukes from livers of slaughtered animals were collected from the abattoirs of aforementioned area. the included 35 samples from infected sheep and 35 samples from 35 infected ca...

Among numerous available genotyping techniques, mass spectrometry (MS) based methods play a major role in providing high quality genotype data at reasonable costs for research and diagnostics, e.g. for pharmacogenetic applications. Ion-pair reversed-phase liquid chromatography hyphenated to electrospray ionization time-of-flight MS (ICEMS) is, for example, a powerful instrument that allows a di...

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial inte...