نتایج جستجو برای: gra8

تعداد نتایج: 16  

2011
Jalal Babaie Mandana Miri Ghazaleh Sadeghiani Mehrak Zare Ghader Khalili Majid Golkar

Diagnosis of Toxoplasma gondii (T.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. Numerous studies have shown that the recombinant production of several toxoplasma antigens, including dense granule antigens (GRAs) has a great potential as diagnostic reagents. Previous studies reported expression of amino terminal GRA8 protein in fusi...

2018
Ye-Ram Kim Jae-Sung Kim Jin-Seung Yun Sojin Kim Sun Young Kim Kiseok Jang Chul-Su Yang

The intracellular parasite Toxoplasma gondii has unique dense granule antigens (GRAs) that are crucial for host infection. Emerging evidence suggests that GRA8 of T. gondii is a promising serodiagnostic marker in toxoplasmosis. However, little is known about the intracellular regulatory mechanisms involved in GRA8-induced host responses. We found that GRA8 interacts with host proteins involved ...

Journal: :Iranian biomedical journal 2009
Jalal Babaie Mehrak Zare Ghazaleh Sadeghiani Mohammad Lorgard-Dezfuli Zohre Aghighi Majid Golkar

BACKGROUND Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. METHODS GRA8 comple...

Journal: :Veterinary Parasitology 2021

Toxoplasma gondii is a worldwide distributed parasite causing abortions and fetal malformations in small ruminants. The aim of this study was to design validate new immunoassay based on the use TgSAG1-GRA8 chimeric antigen for detection anti-T. antibodies serum goats. First, time-resolved fluorescence (TgSAG1-GRA8-TRFIA) developed. In addition, diagnostic performance TgSAG1-GRA8-TRFIA compared ...

Journal: :iranian biomedical journal 0
جلال بابایی jalal babaie مهرک زارع mehrak zare غزاله صادقیانی ghazaleh sadeghiani محمد لرگرد دزفولی mohammad lorgard-dezfuli زهره عقیقی zohre aghighi مجید گلکار majid golkar

background: dense granule antigens (gra antigens) of toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. previous studies reported expression of amino terminal gra8 protein in fusion with large tags such as glutathione-s-transferase. the present study aimed to produce soluble full length immunogenic gra8 in bacteria. methods: gra8 comple...

Ghazaleh Sadeghiani, Jalal Babaie, Majid Golkar, Mehrak Zare, Mohammad Lorgard-Dezfuli, Zohre Aghighi,

Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

Journal: :Clinical and diagnostic laboratory immunology 2005
Klaus-Ingmar Pfrepper Gisela Enders Marion Gohl Doris Krczal Harald Hlobil Doris Wassenberg Erwin Soutschek

To improve serodiagnostic methods for the diagnosis of acute toxoplasmosis during pregnancy, a new test system has been developed and evaluated based on the use of recombinant antigens. Five recombinant Toxoplasma gondii antigens (ROP1, MAG1, SAG1, GRA7, and GRA8) were cloned in Escherichia coli, purified, and applied directly onto nitrocellulose membranes in a line assay (recomLine Toxoplasma)...

Journal: :avicenna journal of medical biotechnology 0

diagnosis of toxoplasma gondii (t.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. numerous studies have shown that the recombinant production of several ttoxoplasma antigens, including dense granule antigens (gras) has a great potential as diagnostic reagents. previous studies reported expression of amino terminal gra8 protein in fus...

Journal: :IOP Conference Series: Materials Science and Engineering 2016

Journal: :The Korean journal of parasitology 2005
Aifen Lin Eun-Hee Shin Tae-Yun Kim Jae-Hwan Park Sang-Mee Guk Jong-Yil Chai

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5o and 3o ends of SAG2, respectively, with...

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