two igm monoclonal antibodies (mab) with strong reactivity for granulocytes and to a lesser extent for lurkat cell lines were established by immunizing balbi c mice with a histiocytic cell line (uy37). these two mabs (designated as 6c9 and 4c4) reacted with blast cells of t-acute lymphoblastic leukemia (t-all) and acute myelogenous leukemia (aml) patients as well as leukemic cells from patients...

Two IgM monoclonal antibodies (MAb) with strong reactivity for granulocytes and to a lesser extent for lurkat cell lines were established by immunizing BALBI c mice with a histiocytic cell line (UY37). These two MAbs (designated as 6C9 and 4C4) reacted with blast cells of T-acute lymphoblastic leukemia (T-ALL) and acute myelogenous leukemia (AML) patients as well as leukemic cells from pat...

To understand the role that hemocytes play in processes of cellular immunity bivalve mollusks, they should be accurately classified based on their morphological and physiological characteristics. The circulating cultured Pacific oyster (Crassostrea gigas) marine mussel (Mytilus galloprovincialis) were investigated using light microscopy flow cytometry. In mussel, two cell types, granulocytes ag...

Neutrophils and eosinophils are granulocytes which characterized by the presence of granules in cytoplasm. Granules provide a safe storage site for granule proteins that play important roles immune function granulocytes. Upon activation, diverse released from into extracellular space contribute to fight against infections. In this article, we describe both neutrophils able kill pathogens review...

ALMOST seventeen billion granulocytes enter and exit the lungs each minute. Although the numbers of granulocytes in pulmonary artery and systemic artery blood are equal under normal conditions, they are not all the same cells, since a sizeable marginated pool of granulocytes in the lungs is constantly exchanging with the pool of circulating cells (Bierman et al., 1952, 1955; Cohen et al., 1979;...

We studied interactions in vitro between human granulocytes and the yeast-like form of Blastomyces dermatitidis, because granulocytes are prominent in the host response to systemic blastomycosis. In Boyden chamber assays, broth culture filtrates of B. dermatitidis contained levels of granulocyte chemotactic activity that were significantly higher than those present in similar culture filtrates ...

OBJECTIVES To characterize the number of granulocytes needed to count on peripheral smear to identify diagnostic anaplasmosis morulae. METHODS Retrospective case study where the peripheral smears of 14 confirmed cases of anaplasmosis were examined. The granulocytes were counted up to 100 and 200 until a morula was identified. The mean counts of three pathologists were calculated to determine ...

Granulocytes collected by reversible adhesion to nylon wool fiber (NWF) function relatively well in standard in vitro tests; however, they have an abnormally shortened survival time in the circulation. Assuming that this rapid disappearance represents clearance and that recognition by phagocytes is important for such clearance, we used an autologous in vitro cell:cell recognition assay to deter...

regulation of normal cell growth and turnover is balanced between cell pro¬liferation, cell differentiation and apoptosis. a disruption of this balance is thought to be an important event leading to carcinogenesis .one of the effector molecules in apoptosis is fas antigen . cross-linking of fas by its ligand (fas l) or agonistic anti fas antibodies induces apoptosis of cells expressing fas on t...

background: accumulating evidence indicates that inflammatory cells migrate into the pancreas tissue and play an important role in the pathogenesis of acute pancreatitis (ap). the aim of this study was to establish a flow cytometric method to enumerate these infiltrating cells in the pancreas of an experimental ap. materials and methods: twelve hours after inducing of ap, mice pancreatic tissue...