background: heat shock protein 70 (hsp70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including leishmania spp. objective: the aim of this study was to clone and express hsp70 from l. infantum strain mcan/ir/96/lon-49 and evaluate antibody response against hsp70 in visceral leishmaniasis (vl). methods: the l. infantum hsp70 gene segment...

aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. the first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. the mainfocus of the present study is to perform the similarit...

introduction: postoperative neurological deficit is the most devastating complication after thoracoabdominal aortic aneurysm repair. despite demonstrated neuroprotective effects of estradiol, its protective efficacy against spinal cord ischemia-reperfusion and underlying mechanisms are not yet elucidated. methods: two groups, each of 10 new zealand white male rabbits, were studied. control grou...

Here we present an in vivo footprinting analysis of the Saccharomyces cerevisiae HSP82 promoter. Consistent with current models, we find that yeast heat shock factor (HSF) binds to strong heat shock elements (HSEs) in non-heat-shocked cells. Upon heat shock, however, additional binding of HSF becomes apparent at weak HSEs of the promoter as well. Recovery from heat shock results in a dramatic r...

background: the aim of this study is to create an ex vivo model to examine the expression of two heat shock protein 70 (hsp70) family members, heat shock protein 72 (hsp72) and heat shock constitute protein 70 (hsc70), at the mrna and protein levels in differentiating corneal cells from air exposed limbal stem cells. materials and methods: limbal biopsies were cultured as explants on a cellular...